Urinary porphyrinogen test (UP)

Porphyrin is derived from bilirubin, which is an intermediate product in the process of heme synthesis in human body. Porphyrin is mainly synthesized in liver and red bone marrow. When certain genetic problems occur, it will cause excessive porphyrin production in human body. To cause hematoporphyrin disease, at this time, the urinary porphyrin can be discharged from the urine through the kidney or the fecal porphyrin can be discharged from the feces through the bile. Hepatic porphyria can be positive, and secondary porphyria caused by poisoning of heavy metals such as lead, arsenic, mercury, zinc or some chemical drugs can also have a positive reaction. When porphyrin is present in the urine, the urine turns red and may also be colorless, but may appear red when exposed to sunlight or acidified to boil. Basic Information Specialist classification: urinary examination classification: urine / kidney function test Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Congenital hematoporphyria (erythropoietic hematoporphyosis), acute hematoporphyria (onset), delayed cutaneous hematoporphyria (onset), lead and heavy metal poisoning, liver disease and some hemolysis Anemia, myocardial infarction, etc. Tips: Use barbital, chlordiazepoxide, methionine, sulfa drugs and phenytoin, griseofulvin and estrogen. Consult a doctor before use. Ban porphyrin-rich foods or drugs before urine testing. In order to avoid decomposition of porphyrin, brown bottles should be used after the specimens are taken, and fresh urine should be sent in time. Normal value Qualitative negative. Recorded as (-). Quantitative 0 ~ 36nmol / 24h (0 ~ 30μg / 24h). Clinical significance Abnormal result Positive (or elevated): congenital hematoporphyria (erythropoietic hematoporphyosis), acute hematoporphyria (onset), delayed cutaneous hematoporphyria (onset), lead and heavy metals Poisoning, liver disease and some hemolytic anemia, myocardial infarction, etc. Need to check the crowd Chronic liver disease patients with hematopoietic dysfunction. Positive results may be disease: hereditary iron granulocyte anemia, delayed skin porphyria considerations Requirements for inspection: 1. In order to avoid decomposition of porphyrin, brown bottles should be used after the specimens are taken, and fresh urine should be sent in time. 2, biliary original instability, easy to change to red and black biliary bilirubin, so be sure to take fresh urine immediately for inspection, immediately check. The sensitivity of this method was 11 mg/L. Due to the large increase in urinary bilirubin in the onset of porphyria, the reaction is dark red, and the urine can be diluted 10 times before detection. Preparation before inspection: 1. Strict and prudent use of barbital, chlordiazepoxide, methyl propylamine (methampine), sulfa drugs and phenytoin, griseofulvin and estrogen. Consult a doctor before use. 2. Disable porphyrin-rich foods or drugs before urine testing. Inspection process Porphyrin is an intermediate product of the synthesis process of hemoglobin, myoglobin, catalase, peroxidase and cytochrome in animals. Commonly used in the human body are coproporphyrin I, III, urinary porphyrin I, III, and protoporphyrin IX. In addition, there is a bile precursor, which is a porphyrin precursor, which is excreted in a large amount in the urine of a certain porphyrin patient. The urine color is normal, but after the urine is left in the sun for a few hours, the urine color deepens and turns reddish brown, which is the result of the biliary porphyrin becoming urinary porphyrin. All kinds of porphyrins are colored substances, and under the ultraviolet light of the special filter, they all show red fluorescence, so they can be quantified by fluorescent colorimetry. The coproporphyrin is dissolved in ether and the urinary porphyrin is insoluble, which is convenient for separation. Various porphyrins have different absorption bands in the spectroscopic spectrum. Not suitable for the crowd Generally no taboos. Adverse reactions and risks No.