hepatitis C virus antibody immunoglobulin G

HCV is the causative agent of hepatitis C. Infection with HCV often leads to chronic hepatitis and cirrhosis, and is associated with liver cancer. HCV is mainly transmitted through the blood, accounting for 90% of hepatitis after transfusion. Anti-HCV examination is the main basis for the diagnosis of hepatitis C. Basic Information Specialist classification: Infectious disease examination and classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Negative means not infected with hepatitis C virus. Positive: Positive indicates infection with hepatitis C virus. Reminder: The positive test is only positive for the initial screening. For some weak positive samples, the test should be repeated or confirmed. Normal value Using a microplate reader, OPD-H2O2 was used as a substrate, and 492 nm; 3,3',5,5'tetramethylbenzidine (TMB)-H2O2 was used at 450 nm. The blank holes are zeroed and the absorbance values ​​of the tubes are read. P/N ≥ 2.1 is positive, otherwise it is negative. Clinical significance Anti-HCV-IgG and IgM antibodies are non-protective antibodies. Most of the acute phase is IgM type, and the chronic phase is mostly IgG type. Its detection is an important indicator for the diagnosis of acute and chronic hepatitis C. In the recovery phase, the anti-HCV is mostly IgG type, and the titer is low. Positive anti-HCV-IgM can confirm early HCV infection. Positive results may be diseases: liver cirrhosis, liver cancer considerations The positive of this test is only positive for the initial screening, and for some weakly positive samples, the test should be repeated or confirmed. Inspection process According to the instructions of the kit, refer to the following method: Add 100 μl of sample dilution to each well, and 5 μl of serum to be tested. At the same time, set Yin, positive and blank control, shake and mix, set at 37 ° C for 20 min, wash 4 times, air dry. Add enzyme-labeled goat anti-human IgG 100 μl at 37 ° C for 20 min, wash 4 times, air dry. Add 1 drop (50 μl) of each of the substrate A and B, mix gently, and let stand at room temperature (15 ~ 30 ° C) for 15 min in the dark. The reaction was terminated by adding 2 mol/L H 2 SO 450 μl. Not suitable for the crowd Those who do not have an indication for examination should not do this check. Adverse reactions and risks Generally no complications and harm.