Urinary 6-keto-prostaglandin F1a

Prostaglandins are a group of unsaturated fatty acids composed of 20 carbon atoms. They were first discovered in semen, hence the name prostaglandin (PG). The PG consists of a five-carbon ring structure and two side chains. Its structure is divided into A, B, C, D, E, F, G, H, I and other types. The Arabic numerals in the lower right corner of the letter indicate the number of double bonds contained in the side chain of the PG molecule, such as PGE1 and PGE2. Substituents on the PG five-carbon ring are marked with α below the ring plane, such as PGF1α, and β above the ring plane, such as PGF2β. Different types of PG have different biological effects. At present, PGE1, PGE2, PGF2α, PGA2, PGI2, TXA2 and TXB2 are the most studied, among which the most widely studied are Pseudocyclin (PGI2) and Thromboxane (TXA2). PG is widely found in various important tissues and body fluids of mammals and humans. It is abundant in blood vessel wall, platelet, lung, kidney, gastrointestinal, brain and reproductive system. The half life of PG is only 1 to 5 min. 6-keto-PGF1α is a stable metabolite of PGI2. PG is a class of hormones that are produced locally and locally. The physiological role of PG is extremely complex and complex, and each type of PG exhibits completely different effects on different tissues and cells. PG has a certain relationship with cardiovascular, reproductive, central nervous, respiratory, digestive, urinary, platelet function, inflammatory response, immune regulation, and tumor metastasis. PGI2 can dilate blood vessels, reduce peripheral vascular resistance, increase organ blood flow, and have a sodium-diuretic effect, which lowers blood pressure. Basic Information Specialist classification: male examination check classification: urine / kidney function test Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Found in patients with chronic renal failure. Normal value: Urinary 6-keto-prostaglandin F1a: 406.9-876.1 pg/min Above normal: No relevant information. negative: Positive: Tips: This method uses high specific radioactivity [3H]-label, using immuno-binding non-equilibrium method, the sensitivity is higher, the minimum detection value is 6.25 ~ 12.5pg / tube. Normal value (1) [3H]-label assay Urine 641.5 ± 234.6pg / min urine. (2) [125I]-labeling method 17 healthy adults, all of whom had no heart, brain and kidney disease, and the test result was 146.94±11.78 ng/L. At the same time, a comparison was performed with the [3H] labeling method. The results of the two methods were statistically processed and there was no significant difference. Clinical significance Changes in 6-keto-PGF1α levels were observed in patients with cardiovascular atherosclerosis with decreased plasma levels of 6-keto-PGF1α, increased TXB2, and similar changes in diabetes and hyperlipidemia. Elevated TXA2/PGI2 ratios tend to cause platelet aggregation, thrombosis, and promote arteriosclerosis and coronary heart disease. The level of 6-keto-PGF1α in the plasma of shock animals caused by bleeding, injury and endotoxin was increased. 2 In patients with chronic renal failure, TXB2 and 6-keto-PGF1α decreased in urine. Intrarenal PG is important for regulating renal blood flow, and there are significant changes in urine in patients with renal vascular hypertension, nephrotic syndrome and Batter sign. 3PG is closely related to the reproductive system, especially the process of ovulation, corpus luteum, corpus callosum, uterine activity, and the operation of eggs and sperm. The increased PGI concentration in pregnant women and the reduced sensitivity to the angiotensin II pressor effect may be associated with increased PGI synthesis in the placenta. 4 Inflammatory reactions such as contact dermatitis in patients with skin eluate PGE and PGF content is 10 times higher than non-inflammatory skin. Inflammatory exudates also contain PGI2 and TXB2. Injection of exogenous PGE and PGI2 and other strong inflammatory reactions such as redness, swelling and heat pain. PG synthetase inhibitors have good anti-inflammatory effects, and also indicate that PG substances play an important role in inflammation. 5 tumor metastasis, PGI2 in the arterial tissue of patients with malignant tumors is less than that of benign tumor patients, and the half-life is shortened. The production of PGI2 and TXB2 may prevent tumor cells from invading platelets and adhering to the surface of blood vessels. Factors that inhibit platelet TXA2 production and increase vascular endothelial cell PGI2 production are tumor metastasis. Low results may be diseases: acute glomerulonephritis, acquired platelet dysfunction, diabetic nephropathy (1) This method uses a high specific radioactivity [3H]-label, using immuno-binding non-equilibrium method, the sensitivity is high, the minimum detection value is 6.25 ~ 12.5pg / tube. (2) 6-keto-prostaglandin F1a100, 500 pg/ml was added to normal plasma, and the recovery rate was 88.5% to 98.5%, the intra-assay CV was 5.5% to 10.5%, and the inter-assay CV was 10.5% to 15.1%. Inspection process [3H]-label assay. Not suitable for the crowd No taboos. Adverse reactions and risks No.