Phenylalanine deaminase test

Phenylalanine deaminase test means that if the bacteria have phenylalanine deaminase, the phenylalanine in the medium can be deaminated to phenylpyruvate, and the keto acid can change the ferric chloride indicator. It is green. Proteus and Provideinia and Moraxella have phenylalanine deaminase activity. The phenylalanine deaminase produced by the bacteria deaminates the phenylalanine to form phenylpyruvate, and the addition of the ferric chloride reagent produces a green reaction. If you extend the time, it will cause discoloration. Basic Information Specialist Category: Infectious Diseases Inspection and Classification: Urine/Kidney Function Examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Other bacteria in the Enterobacteriaceae family are negative. Positive: Proteus, Providencia, and Morganella are all positive. Tips: Pay attention to the hygiene of the urethra. Normal value The type and proportion of the flora in the body are normal, and the human body is in a dynamic balance. Clinical significance Abnormal results: Proteus, Provided, and Morganella were positive, and other bacteria in Enterobacteriaceae were negative. Urinary tract infections and nosocomial infections caused by Providencia. Nosocomial infections and urinary tract stones caused by Provenus. Nosocomial infection, urinary tract infection, and diarrhea caused by Morganella. People who need to be examined: those with the above symptoms. Positive results may be diseases: precautions for urinary tract infections Inappropriate crowd: No. Taboo before inspection: pay attention to work and rest, normal diet, health habits Requirements for inspection: Actively cooperate with the doctor. The phenylalanine deaminase produced by the bacteria deaminates the phenylalanine to form phenylpyruvate, and the addition of the ferric chloride reagent produces a green reaction. If you extend the time, it will cause discoloration. Inspection process Phenylalanine deaminase test Method: Inoculate a large amount of the test bacteria in the culture solution obtained by the test part into phenylalanine medium, incubate at 35 ° C for 18~24h, add dropwise 4~5 drops of 100g/L ferric chloride reagent, observe immediately There is no green color at the colony growth. Result: Green appeared positive. Not suitable for the crowd There are no taboos. Adverse reactions and risks No.