Molecular Hybridization of Nucleic Acids

Molecular hybridization of nucleic acids is a powerful tool for qualitative or quantitative detection of specific RNA or DNA sequence fragments. It was developed using the principle of base complementation of nucleic acid molecules. Under conditions such as heating or addition of a denaturant in an alkaline environment, hydrogen bonds between double-stranded DNA are disrupted (denatured), and the double strands are untwisted into two single strands. At this time, heterologous DNA or RNA (single-stranded) is added and incubated at a certain ionic strength and temperature (refolding). If certain regions between heterologous DNA or RNA have complementary base sequences, then renaturation Hybridized nucleic acid molecules can be formed. Basic Information Specialist Category: Oncology Inspection Category: Genetic Testing (DNA) Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Negative means normal. Positive: Positive indicates the risk of fatty liver, hyperlipidemia, cardiovascular and cerebrovascular diseases and tumors. Tips: Pay attention to normal eating habits before check, pay attention to personal hygiene. Normal value The type and proportion of the flora in the body are normal, and the human body is in a dynamic balance. Clinical significance To screen for chronic diseases such as fatty liver, obesity, hyperlipidemia, and cardiovascular disease caused by long-term intense work of white-collar workers, to identify potential hazards to health risks early, and to propose comprehensive health improvement programs. Abnormal results of various pathogenic microbial infections and various unhealthy symptoms. The people in need of examination are those with various pathogenic microorganisms, tumors or cancer patients. Precautions Inappropriate crowd: No. Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Requirements for inspection: Actively cooperate with the doctor. Inspection process (1) rRNA-DNA hybridization: When denatured rRNA is mixed with denatured DNA, rRNA forms a hybrid double strand with its complementary DNA strand, and when the rRNA molecule hybridizes with the heterologous DNA, it can also form a complementary double strand in its homologous region. The stability of the hybrid double strand is positively correlated with its homology and is suitable for the classification of bacterial genus and genus. The most commonly used method is the nitrocellulose membrane bonding method. (2) Nucleic acid probe technology: The use of nucleic acid probe technology to detect pathogenic microbial nucleic acids is a major development in clinical diagnostics. The principle is to use known sequence-specific DNA fragments (called probes) labeled with enzymes, chemiluminescent substances, radionuclides or biotin. Under certain conditions, the probe hybridizes with the nucleic acid in the sample to be tested according to the principle of base complementation, and the hybridization signal is detected to identify the presence or absence of the corresponding pathogenic microorganism gene and its molecular size. Commonly used nucleic acid probe techniques are solid phase hybridization (dot hybridization, in situ hybridization, Southern blotting, Northern blotting, etc.) and liquid phase hybridization techniques. Nucleic acid probes are suitable for directly detecting pathogenic microorganisms in clinical specimens, and are not affected by non-pathogenic microorganisms. Therefore, it is of great significance for the detection of microorganisms that are not isolated or cultured or difficult to isolate and culture. With the continuous improvement of the probe label, the detection kit is commercialized and the operation is simpler and easier. Not suitable for the crowd Taboo people: No. Adverse reactions and risks It does not generally cause special complications and hazards.