Cerebrospinal fluid apolipoprotein A1, B100, CⅡ, CⅢ, E

The special protein that makes up lipoproteins is called Apo. Apo is the major protein component that determines the properties of lipoproteins. Among different lipoproteins, the type, content and function of Apo are also different. The main functions of Apo are: 1 constitute a lipoprotein, making plasma lipids soluble. 2 Activate or inhibit enzymes involved in lipoprotein metabolism. 3 recognizes lipoprotein receptors and binds to specific lipoprotein receptors. 4 Binding and transporting lipids, stabilizing lipoprotein structure and the like. Basic Information Specialist classification: examination classification: cerebrospinal fluid examination Applicable gender: whether men and women apply fasting: fasting Tips: The examinee should stop taking epinephrine, isoproterenol, meperidine, nicotine, aspirin and other drugs, and maintain a reasonable diet and rest. Normal value (1) ApoB100 and CII were not detected by one-way immunodiffusion method, while ApoCIII content was extremely small; ApoA1 was 0.01±0.0054 g/L, and ApoE was 0.0069±0.0016 g/L and 22.2%. (2) Rocket immunoelectrophoresis ApoA10.04±0.01g/L. (3) ELISA method ApoE0.008 ± 0.001 g / L. Clinical significance The apolipoprotein content in cerebrospinal fluid is much lower than that in blood. Immunohistochemistry experiments show that astrocytes and some neurons in the frontal lobe of the brain can synthesize ApoE, while ApoA1 and ApoCII in CSF may infiltrate from the blood. However, when suffering from a nervous system disease, the blood-brain barrier function may be impaired. Increased permeability, ApoA1 in the blood can be more infiltrated into CSF, ApoA1 content increases; because ApoB100 has a large molecular weight, meningitis, cerebrovascular disease, ApoA1, ApoE in cerebral cysticercosis are elevated in CSF . The determination of apolipoproteins can provide valuable information for the diagnosis of certain neurological diseases. High results may be diseases: meningitis, cerebral cysticercosis matters needing attention (1) Apo(a) and apoB in LP(a) particles are linked by disulfide bonds; the amino acid composition of apo(a) is highly homologous to PMG, so the antisera obtained by immunizing animals with LP(a) ( The polyclonal antibody has both anti-apoB and PMG antibodies, and is usually purified by affinity chromatography, and it is difficult to obtain pure apo(a) antibody. However, McAb prepared by hybridoma technology can avoid cross-reactivity with apoB and PMG. (2) In order to avoid the difference between the holes and reduce the error, it is best to do 2 to 3 copies of the same specimen in parallel and take the average value. (3) The precision of dilution should be strictly controlled. Since the serum LP(a) level can be less than 10 mg/L or up to 1000 mg/L or more, the specimens that are too high or too low should be adjusted and then re-measured. (4) The coated antibody and the enzyme-labeled antibody cannot be repeatedly thawed, and the dilution/blocking solution and the substrate solution should be freshly prepared. Increased blank absorbance may be due to insufficient or obsolete substrate solution. (5) The CV of the method is <5%, and the inter-assay CV is <9%. The experimental error sources are inter-plate difference, inter-well difference, dilution and loading error, reagent quality and incubation time. Inspection process (1) One-way immunodiffusion method. (2) Rocket immunoelectrophoresis. (3) ELISA method. Not suitable for the crowd 1. If there is obvious papilledema or cerebral palsy, contraindications are contraindicated. 2. Patients who are in shock, exhaustion or endangered state and local skin inflammation, and lesions in the posterior cranial fossa are contraindicated. Adverse reactions and risks If the patient has symptoms such as breathing, pulse, or abnormal color during puncture, stop the operation immediately and deal with it accordingly.