Amoeba antigens and antibodies

Amoeba protozoan antigens and antibodies are protozoal antigens and antibodies in the blood after detection of E. histolytica in the human intestinal tract. The amoeba antigen in feces and liver pus is often detected by direct fluorescence method; the amoeba antibody in serum is detected by enzyme-linked immunosorbent assay and indirect immunofluorescence assay. Basic Information Specialist classification: Digestive examination classification: pathogenic microbiological examination Applicable gender: whether men and women apply fasting: fasting Including items: amoeba protozoan antigen, amoeba protozoan antibody Tips: Before the examination, the diet is light and alcohol is prohibited. Check for an empty stomach in the morning. Normal value 1. Negative enzyme-linked immunosorbent assay. 2. Indirect immunofluorescence is negative. 3. Negative direct immunofluorescence. Clinical significance Positive in amebic liver abscess, intestinal amebiasis or worms. Positive results may be diseases: cecal amoebic granuloma, amoebic bowel disease, chronic amebiasis enteritis, acute amoebic dysentery precautions The indirect fluorescent antibody test is highly sensitive and specific, mostly using pathogens as antigens, and is intuitive and can be used for the diagnosis of various parasitic diseases. Inspection process 1. Principle of determination of amoeba antigens and antibodies The principle of immunofluorescence technology is to use the property that the material absorbs light energy to produce an excited state and emit light. Fluorescence (fluorescein isothiocyanate or rhodamine B200) having such a property is chemically bonded to a specific antibody (antigen) molecule without impairing the activity of the antibody or antigen under a fluorescence microscope Tracer technology. The binding of antibodies to antigens is highly specific, so as long as one of these factors is known, another factor is known. The immunofluorescence technique uses these two characteristics to react an invisible antigen-antibody reaction (primary antibody) with a fluorescein-labeled antibody (anti-antibody) to become visible to the naked eye, and then according to histology and cytology. To determine where the specific fluorescence is. The bright green fluorescence we can see under a fluorescence microscope is not emitted by the specimen itself (colorless), but by the bright green fluorescence emitted by the fluorescent material. Fluorescently labeled antibody technology is called fluorescent antibody technology, and fluorescently labeled antigen technology is called fluorescent antigen technology. 2, reagents (1) Fluorescently labeled antibodies (or antigens). (2) 0.01 mol/L pH 7.2 PBS buffer. (3) Tissue or cell matrix tablets. (4) Buffered glycerol (pH 7.2). (5) Fluorescence microscope. (6) Wet box. 3, the operation method (1) Antigen (or antibody) matrix tablets (various tissue frozen sections or cultured cells, etc.) The matrix pieces are taken out from the refrigerator and immediately air-dried. Before use, it is treated with anhydrous ethanol or a fixing agent such as acetone or methanol (the fixing agent is selected according to the experimental requirements). Generally fixed for 3 to 15 minutes, dry at room temperature. (2) The test substance (serum, cerebrospinal fluid or other exudate, etc.), if necessary, diluted with PBS (1:5 or 1:10) onto the antigen matrix sheet, and placed in a thermostat at room temperature or 37 ° C for 30 min to 1 h. . (3) Flush for 15 min with 0.01 mol/L pH 7.2 PBS (replace PBS 3 times). (4) Add fluorescently labeled antibody and set at room temperature or 37 ° C incubator for 30 min to 1 h. (5) PBS rinse with (3). (6) Buffered glycerin (pH 7.2) was mounted and examined by fluorescence microscopy. Not suitable for the crowd Those who do not have an indication for examination should not do this check. Adverse reactions and risks Generally no complications and harm.