TORCH inspection

TORCH refers to a group of pathogens TO refers to Toxoplasma gondii, R refers to rubella virus, C refers to cytomegalovirus, H refers to herpes simplex virus. This test is often used as a routine examination item for genital tract infections in women during pregnancy. If infected, it will cause miscarriage, premature delivery, intrauterine death, fetal hydrocephalus, neurodevelopmental disorders, congenital heart disease, etc., for prenatal and postnatal care and habituation. The cause analysis of abortion has reference value. Basic Information Specialist classification: maternal checkup classification: pathogenic microbiological examination Applicable gender: whether women are fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Negative means normal, not infected with rubella, giant cells and other viral infections. Positive: Found in Toxoplasma gondii, rubella virus, cytomegalovirus, herpes simplex virus and other viral infections. Tips: Pregnant women should be tested for TORCH during the first trimester. Normal value Negative: (P/N is less than 2.l). Clinical significance Positive: see Toxoplasma gondii, rubella virus, cytomegalovirus, herpes simplex virus and other viral infections. This test is often used as a routine check for genital tract infections in women during pregnancy. Toxoplasma infection Toxoplasma infection is a zoonotic disease in which cats and other animals are the source of infection. Acquired light infection is often asymptomatic, but antibodies can be found in serum; severe cases can cause various symptoms, such as high fever, muscle or joint pain, lymphadenopathy, etc.; intrauterine infection through the placenta can cause stillbirth, premature birth, birth It can manifest a series of central nervous system symptoms as well as congenital damage to the eyes and internal organs. 2. Rubella virus Pregnant women infected with rubella often in the first l to 6 weeks of pregnancy, in addition to causing miscarriage, death, the birth of infants can also occur congenital rubella syndrome. A positive lgM antibody indicates a recent infection and should be terminated if necessary. 3. The cytomegalovirus lgM antibody positive indicates that the patient has a recent cytomegalovirus infection, but should be analyzed in combination with clinical conditions. 4. Herpes simplex virus Mainly cause herpetic stomatitis, eczema herpes, herpetic keratoconjunctivitis, neonatal herpes, herpetic vulvovaginitis. Infections other than the reproductive organs are mostly caused by herpes simplex virus-type I, and infections of the reproductive organs are mostly caused by the simple herpesvirus-type II. This test does not distinguish between herpes simplex virus type I or herpes simplex virus type II. Positive lgM antibodies suggest a recent herpes simplex virus infection. Positive results may be diseases: congenital rubella, rubella, cytomegalovirus disease, herpes simplex virus infection precautions Pregnant women should undergo TORCH examination during the first trimester. Inspection process (1) Titration of complement and hemolysin. 1 Take 1ml of complement, add 9ml of BBS, and then dilute to 1:20, 1:30, 1:40, 1:50, 1:60, 1:80, 1:100, 1:200, 1:400, and take 50 0.2 ml of hemolysin, add 9.8 ml of BBS, which is 1:100 hemolysin, and then continue to dilute to 1:800, 1:1600, 1:3200, 1:6400. 2 Take the square array of test tubes, longitudinally add 0.1ml of different concentrations of complement, and add 0.1ml of hemolysin in each row (all should be added from the highest dilution), then add BBS0.2ml, complement and The hemolysin control tubes were each added with BBS 0.3 ml, and finally 2% sheep red blood cells were added to each tube. The total amount of each tube was 0.5 ml, shaken, placed in a 37 ° C water tank for 30 min, and the results were observed. The highest dilution of complete hemolyzed complement and hemolysin is the respective unit. In the above table (Table 1), hemolysin 1:3200 is a hemolysin unit, and complement 1:80 is a complement unit. In practical applications, the hemolysin is 2u (3200÷2=1600) and the complement is 2.5u (80÷2.5=32). (2) Formal test: 1 Operate with a V-type microplate, each specimen was made into one test well and one serum control well. BBS0.025ml was added to each well. 2 Dip serum (0.025 ml) with a dilution stick to the first well, rotate and transfer to the second well for a total of eight wells, thus obtaining a 1:2 to 1:256 dilution. (3) Judgment of results: The results of each tube of the control group should meet the following conditions, otherwise it should be redone. Complement control tube: 2.5u = 0, 1u = 0 ~ ±, 0.5u = 3 ~ 4. Sheep red blood cell control tube: 4. Antibody control tube: 0. Negative control tube; 1:2 to 1:256=0. Positive control tube: the same positive serum, the results should be consistent. When the reaction tube of each measuring tube is the strongest, one tube is still ± report suspicious, and 1 to 4 reports positive. If both the measuring tube and the serum control tube are anti-complement, all the measuring tubes are 0, and the report is negative. The method of examining the antibody is the same as the method of examining the antigen, except that four units of known antigen are used instead to determine the serum to be tested. Not suitable for the crowd Those who do not have an indication for examination should not do this check. Adverse reactions and risks Generally no complications and harm.