Ethanol gel test

Due to the presence of dissociated fibrin in plasma during disseminated intravascular coagulation, fibrin early degradation products form a complex with fibrin monomers, fibrin monomers can be released by 50% ethanol dissociation, and can be self-polymerized. Fibrin jelly and can appear within 1 min. Basic Information Specialist classification: examination classification: blood examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: Negative is normal. Positive: Positive indicates diffuse intravascular coagulation. Tips: People with obvious bleeding tendency should not do this check. Normal value negative. Clinical significance Positive diffuse intravascular coagulation (DIC). 50% ethanol can separate the complex formed by FM and FDP, and the isolated FM can self-aggregate to form jelly-like solidification or protein filament. Its diagnostic significance is the same as the 3P test, but the positive rate is lower than the 3P test, and the specificity is higher than the 3P test. In DIC, gel formation occurs within 1 min, whereas primary fibrin is negative when dissolved. Positive results may be diseases: cardiogenic shock, disseminated intravascular coagulation considerations (1) The temperature in the experiment is preferably higher than 20 °C. (2) When the final concentration of ethanol is 10% to 15%. The formation of jelly is best. (3) This test is affected by the fibrin content, but is not interfered by FDP and heparin-like substances. (4) The false positive result can be eliminated by adding 0.1 mol/L NaOH 1 drop, and the fibrin polymer can be dissolved without being dissolved by NaOH but not specifically precipitated. (5) The positive rate of this test is lower than the 3P test, but the specificity is higher than the 3P test. Inspection process (1) Take 0.5 ml of PPP with 1:9 anticoagulated sodium citrate. (2) Add 0.15 ml of 50% ethanol solution and shake gently. (3) The results were observed after standing at room temperature (18-28 ° C) for 10 min. Not suitable for the crowd Taboo population: People with a significant tendency to bleed. Adverse reactions and risks This test requires blood collection, so it may damage the blood vessels. People with coagulation disorders or bleeding tendency should not perform this test.