CSF protein electrophoresis

Cerebrospinal fluid protein electrophoresis is mainly used for the diagnosis of multiple sclerosis, neurosyphilis and subacute sclerosing panencephalitis. Basic Information Specialist classification: examination classification: cerebrospinal fluid examination Applicable gender: whether men and women apply fasting: fasting Tips: Due to the low protein content of cerebrospinal fluid, it must be concentrated before electrophoresis. Normal value Filter paper method: Albumin 0.55～0.69 (55%～69%); Globulin α 10.03～0.08 (3%～8%); 2020.04～0.09 (4%～9%); Β0.10～0.18 (10%～18%); γ 0.04 to 0.13 (4% to 13%). Clinical significance (1) albumin increases spinal canal obstruction, brain tumors, and partial vascular diseases. (2) Increase in α1 globulin is seen in brain infection, acute bacterial meningitis, polio; reduction is seen in the acute phase of brain trauma. (3) α2 globulin increases brain metastases, cancerous meningitis, glioma, and cerebellopontine angle tumors. (4) β-globulin increased multiple sclerosis, subacute sclerosing panencephalitis, tremor palsy, brain atrophy, Alzheimer's disease, acromegaly, amyotrophic lateral sclerosis, multiple nerve roots Inflammation, facial nerve palsy, diabetic peripheral neuritis, brain tumor (glioma), epilepsy, pseudo brain tumor, etc. (5) gamma globulin increases multiple sclerosis, subacute sclerosing panencephalitis, viral encephalitis, brain abscess, multiple radiculitis, alcohol-toxic peripheral neuritis, plasmacytoma, glioma, Cerebellopontine angle tumor, brain trauma, sarcoidosis, increased serum gamma globulin (cirrhosis, connective tissue disease, multiple myeloma). High results may be diseases: polio, Alzheimer's disease, sarcoidosis, brain abscess, mixed connective tissue disease considerations Because the protein content of cerebrospinal fluid is small, it must be concentrated before electrophoresis. Generally, dialysis method is used, and the dialysate can be made of high molecular weight polyethylene glycol and dextran. In recent years, high-performance capillary electrophoresis has been used with higher resolution and the cerebrospinal fluid does not need to be concentrated. Inspection process A cellulose acetate membrane process was used. Not suitable for the crowd 1. If there is obvious papilledema or cerebral palsy, contraindications are contraindicated. 2. Patients who are in shock, exhaustion or endangered state and local skin inflammation, and lesions in the posterior cranial fossa are contraindicated. Adverse reactions and risks If the patient has symptoms such as breathing, pulse, or abnormal color during puncture, stop the operation immediately and deal with it accordingly.