Cerebrospinal fluid IgG/albumin ratio

When the central nervous system develops lesions (such as infection, inflammation, tumor, trauma, hemorrhage, edema, etc.), the chemical components in the cerebrospinal fluid may change, and can be used as a clinical diagnosis and treatment by measuring changes in certain chemical components in the cerebrospinal fluid. The basis for disease and prognosis observations. Cerebrospinal fluid protein composition changes are one of them. Basic Information Specialist classification: examination classification: cerebrospinal fluid examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: No relevant information. Normal value: Cerebrospinal fluid IgG/albumin ratio: 0.3-0.7 Above normal: Found in multiple sclerosis. negative: Positive: Tips: The key to this method is to keep the antibody in excess, but not too thick, so as not to waste the antiserum. Normal value 0.3 to 0.7. Clinical significance Elevation is seen in multiple sclerosis. High results may be diseases: pediatric haemophilic influenza bacillus meningitis, multiple sclerosis, multiple sclerosis associated with uveitis considerations The key to this method is to keep the antibody in excess, but not too thick, so as not to waste the antiserum. The test should be blank, generally better by self-control, that is, the turbidity is measured once after adding the specimen, followed by adding anti-serum and re-testing after incubation, and subtracting the original measured value from the re-test result. Because the amount of Ig in patients with immunoglobulin proliferative disease is extremely high, there may be an excess of antigen during the measurement. At this time, an equal amount of diluted reference serum may be added after the turbidity, such as a large increase in turbidity is normal; if the turbidity is not increased or If it falls, it indicates that the antigen is excessive. The specimen to be tested should be diluted and retested. Inspection process The method is as follows: adding 0.9 ml of turbid liquid to the test tube, followed by adding 1:40 to 1:800 specimen or standard (reference) dilution (IgA, IgM, C3, etc. is 1:40, IgG is 1:200, ALB is 1) : 800) 50μl, shake well; add antibody dilution (predetermined according to antiserum titer) 50μl, shake well; place at 37 ° C for 20 ~ 30min, then measure A420nm value, and according to the known different content of the standard as a reference curve, from Isolation of unknown samples. Not suitable for the crowd 1. If there is obvious papilledema or cerebral palsy, contraindications are contraindicated. 2. Patients who are in shock, exhaustion or endangered state and local skin inflammation, and lesions in the posterior cranial fossa are contraindicated. Adverse reactions and risks If the patient has symptoms such as breathing, pulse, or abnormal color during puncture, stop the operation immediately and deal with it accordingly.