Glycated hemoglobin fraction (GHb, HbA1c)

Glycated hemoglobin (GHb) refers to the part of hemoglobin in the blood that is bound to glucose. When the blood glucose concentration is high, the glycated hemoglobin content formed by the human body will be relatively high. The life span of red blood cells in humans is generally 120%. Before the cells die, the glycated hemoglobin content in the blood will remain relatively unchanged. Because of the saccharification, the erythrin level reflects the average blood glucose level within 120 days before the test, and it has nothing to do with whether the blood is taken, whether the patient is fasting, whether insulin is used or not. It is a good indicator for determining long-term control of diabetes. Basic Information Specialist classification: growth and development check classification: endocrine examination Applicable gender: whether men and women apply fasting: not fasting Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. Normal value (1) Ion exchange column chromatography: 6.1% to 7.9% of total hemoglobin. (2) Agarose electrophoresis: 5% to 7.5% of total hemoglobin. (3) Thiobarbituric acid colorimetric method: 129 to 142 μmol/mol Hb. (Note the specific reference value depends on each laboratory.) Clinical significance Reducing the lack of hereditary growth hormone receptors found in growth hormone deficiency and high growth hormone levels can also be seen in cachexia, severe malnutrition, and severe liver disease. Elevated in giant disease and acromegaly. Low results may be diseases: high results of acromegaly may be diseases: growth hormone deficiency, giant disease precautions First, the precautions before blood draw: 1, take venous blood, no need to fasting before blood draw. 2, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Second, after blood draw should pay attention to: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Inspection process The test tube is added with 0.1 ml of serum (plasma) to be tested, and 0.1 ml of distilled water is added to the blank tube, and 4 ml of NBT reagent pre-warmed at 37 ° C is added, mixed, placed in a 37 ° C water bath for 15 min, immediately taken out, and cooled by running water (less than 25 ° C). ). After cooling for 15 minutes, the wavelength of the spectrophotometer is 550 mm, and the 10 mm optical path is zeroed by a blank tube, and the absorbance of the measuring tube is read. The results were found from the standard curve. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Generally not.