Anti-insulin antibody (AIAb)

Insulin-dependent diabetes mellitus (1DDM) is a disorder characterized by metabolic disorders due to an absolute deficiency in insulin secretion, and requires a large amount of exogenous insulin to maintain life. Anti-insulin antibodies (AIAbs) are produced by the injection of exogenous insulin, and even intermittent use can often cause reminiscent antibody responses. The methods for detecting AIAb include ELISA method, immunospot method, etc. The procedure of ELISA method is introduced to purify human, pig or bovine crystal insulin, and the serum to be tested is diluted by l10, and the optimal concentration of HRP labeled anti-human is added. IgC antibody or labeled F(ab') of anti-human IgA, with substrate coloration, positive results were tested for the highest dilution of serum AIAb. Basic Information Specialist classification: examination classification: endocrine examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Dependent diabetic patients, hyperthyroidism patients. Tips: Keep a normal mindset. Normal value Normal human serum anti-insulin antibodies are negative. (Note the specific reference value depends on each laboratory.) Clinical significance Normal human serum anti-insulin antibodies are negative. The significance of measuring insulin antibodies is: (1) High titer insulin antibodies in the therapeutic cycle of insulin-dependent diabetic patients are the main cause of severe insulin resistance. Detection of A-IAb can guide the dosage of insulin and provide a basis for the treatment of drug-resistant diabetes. When the antibody titer is high, the fast-acting insulin can be appropriately increased. When the antibody titer is low, the long-acting insulin is used instead. (2) Judging insulin-dependent diabetes prognosis The insulin release curve is low and the insulin antibody titer is high, indicating that the patient is not islet failure, suggesting that the condition is stable. On the contrary, it indicates insulin failure and has a poor prognosis. (3) Anti-insulin antibodies can also be detected in patients with hyperthyroidism. Positive results may be diseases: precautions for hyperthyroidism in the elderly Pre-examination contraindications: Anti-insulin antibodies are produced by injection of exogenous insulin, and even intermittent use can often cause reminiscent antibody reactions. Patients should draw attention in this regard Requirements for inspection: (1) Judging the prognosis of insulin-dependent diabetes: the insulin release curve is low and the insulin antibody titer is high, indicating that the patient is not islet failure, suggesting that the condition is stable. On the contrary, it indicates insulin failure and has a poor prognosis. (2) Anti-insulin antibodies can also be detected in patients with hyperthyroidism. (3) When the serum to be tested contains anti-insulin antibodies at the same time, the results of the anti-insulin receptor antibody (IRA) are interfered with and need to be corrected by other methods. Inspection process How to detect anti-insulin antibodies? Insulin-dependent diabetes mellitus is caused by a lack of insulin secretion, which is caused by metabolic disorders, and requires a large amount of exogenous insulin to maintain life. Anti-insulin antibodies are produced by the injection of exogenous insulin, and can often cause reminiscent antibody reactions even when used intermittently. The method for detecting insulin antibodies includes an ELISA method, an immunospot method, and the like, and an ELISA method is now described. We can use purified human, pig or bovine crystalline insulin for coating, the test serum is diluted 1:10, plus the optimal amount of working concentration of HRP-labeled anti-human IgC antibody or labeled anti-human IgA insulin antibody, plus substrate Color development, positive results were followed by the highest dilution of serum insulin antibody b. Not suitable for the crowd There are generally no taboos. Adverse reactions and risks Generally not.