Adrenocorticotropic hormone (ACTH)

Corticotropin is a micro-peptide hormone secreted by the pituitary gland and is a major regulator of adrenal cortex activity. All reagents except the precipitant should be equilibrated at room temperature before use. Basic Information Specialist classification: growth and development check classification: endocrine examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Found in pituitary dysfunction, adrenal cortical tumors, pituitary tumors, pituitary damage. Normal value: ACTH (8 am): 2.2-17.6 pmol/L ACTH (4 pm): 1.1-8.8 pmol/L Above normal: Found in stress (such as burns, surgery, hypoglycemia, etc.), primary adrenal insufficiency, Cushing's syndrome, congenital adrenal hyperplasia, pituitary adrenocortical hormone cell tumor. negative: Positive: Tips: After 8 pm on the day before the medical examination, you should start fasting for 12 hours, so as not to affect the test results. Normal value 8:00 am to 17.6 pmol/L. At 4 o'clock in the afternoon, 1.1 to 8.8 pmol/L. Clinical significance Increased in stress, primary adrenal insufficiency, Cushing's syndrome, Nelson syndrome, congenital adrenal hyperplasia, pituitary adrenocortical hormone cell tumor. Decreased in pituitary dysfunction, adrenal cortical tumors, pituitary tumors, anterior pituitary damage. Low results may be diseases: pediatric adrenal insufficiency, adult total pituitary dysfunction, pediatric Cushing's syndrome, secondary adrenal insufficiency, primary adrenal insufficiency results may be disease: adrenal crisis, brain Pituitary adenoma, endocrine hypertension, Xi'an syndrome considerations First, the precautions before blood draw 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Second, should pay attention after blood draw 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. 3. Please inform the doctor about the recent medication and special physiological changes before the test. Inspection process All reagents except the precipitant should be equilibrated at room temperature before use. (1) Mark T (total count), NSB (non-specific binding), A (maximum binding) and B to G, indicating quality control and blood samples, all for double tube. (2) Add 100 μl of 0 standard to NSB and A tube, add 100 μl of corresponding reagent to B to G tube, and add 100 μl to both quality control and blood samples. (3) Add 100 μl of ACTH antiserum (except NSB and T tube) to each tube and mix. (4) Incubate for 60 min at room temperature. (5) 100 μl of ACTH marker was added to each tube. (6) Incubate at 4 ° C for at least 16 h. (7) Add 1 ml of a precipitant to each tube and mix. (8) Centrifuge at 3000 x g for 15 min. (9) The rack was tilted with a foam, the supernatant was poured, and the precipitate was retained for counting. (10) 1 min count. Note: Place the tube on the absorbent paper for 10 min, gently tap the tube to remove any remaining droplets. Not suitable for the crowd People with reduced hematopoietic function such as leukemia, various anemia, myelodysplastic syndrome, or people with thrombocytopenia should pay attention to blood draw, and should not take more or more blood. Adverse reactions and risks 1. After the blood is drawn, do not press the needle hole to avoid subcutaneous hematoma. If there is a small piece of bruise in the blood, it is slightly tender, please don't panic, you can do hot compress after 24 hours to promote the absorption of blood. The general small amount of congestion will gradually absorb in 3 to 5 days and the color will become lighter and return to normal. 2. After the blood draw, symptoms such as dizziness, vertigo, fatigue, etc. should be immediately supine, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved.