adrenocorticotropic hormone

Corticotropin is a micro-peptide hormone secreted by the pituitary gland and is a major regulator of adrenal cortex activity. The secretion of adrenocorticotropic hormone is controlled by the corticotropin releasing hormone, which is secreted by the hypothalamus and responds to low concentrations of cortisol and stress. Normal adrenocorticotropic hormone secretion has the same circadian rhythm as cortisol, high in the morning, low in the afternoon and evening. Determination of adrenocorticotropic hormone levels is primarily used for the differential diagnosis of primary and secondary adrenal insufficiency. Basic Information Specialist classification: growth and development check classification: endocrine examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Found in pituitary dysfunction, adrenal cortical tumors, pituitary tumors, pituitary damage. Normal value: ACTH (8 am): 2.2-17.6 pmol/L ACTH (4 pm): 1.1-8.8 pmol/L Above normal: Found in stress (such as burns, surgery, hypoglycemia, etc.), primary adrenal insufficiency, Cushing's syndrome, congenital adrenal hyperplasia, pituitary adrenocortical hormone cell tumor. negative: Positive: Tips: When analyzing the results, pay attention to the influence of physiological factors, such as exercise, excitement, hunger, etc., can increase the secretion of adrenocorticotropic hormone. Normal value 8 am: 2.2 ~ 17.6pmol / L. 4 pm: 1.1 ~ 8.8pmol / L. Clinical significance Reduced in pituitary dysfunction, adrenal cortical tumors, pituitary tumors, pituitary damage. Elevated in stress (such as burns, surgery, hypoglycemia, etc.), primary adrenal insufficiency, Cushing's syndrome, congenital adrenal hyperplasia, pituitary adrenocortical hormone cell tumor. The result is low, the disease may be high: the result of pediatric adrenal insufficiency is high. Possible diseases: chronic adrenal insufficiency, Cushing's syndrome (1) When high-dose glucocorticoids are treated, the results of adrenocorticotropic hormone measurement are also reduced. (2) When analyzing the results, attention to the effects of physiological factors, such as exercise, excitement, hunger, etc., can increase the secretion of adrenocorticotropic hormone. Inspection process All reagents except the precipitant should be equilibrated at room temperature before use. (1) Mark T (total count), NSB (non-specific binding), A (maximum binding) and B to G, indicating quality control and blood samples, all for double tube. (2) Add 100 μl of 0 standard to NSB and A tube, add 100 μl of corresponding reagent to B to G tube, and add 100 μl to both quality control and blood samples. (3) Add 100 μl of ACTH antiserum (except NSB and T tube) to each tube and mix. (4) Incubate for 60 min at room temperature. (5) 100 μl of ACTH marker was added to each tube. (6) Incubate at 4 ° C for at least 16 h. (7) Add 1 ml of a precipitant to each tube and mix. (8) Centrifuge at 3000 x g for 15 min. (9) The rack was tilted with a foam, the supernatant was poured, and the precipitate was retained for counting. (10) 1 min count. Note: Place the tube on the absorbent paper for 10 min, gently tap the tube to remove any remaining droplets. Not suitable for the crowd People with reduced hematopoietic function such as leukemia, various anemia, myelodysplastic syndrome, or people with thrombocytopenia should pay attention to blood draw, and should not take more or more blood. Adverse reactions and risks 1. After the blood is drawn, do not press the needle hole to avoid subcutaneous hematoma. If there is a small piece of bruise in the blood, it is slightly tender, please don't panic, you can do hot compress after 24 hours to promote the absorption of blood. The general small amount of congestion will gradually absorb in 3 to 5 days and the color will become lighter and return to normal. 2. After the blood draw, symptoms such as dizziness, vertigo, fatigue, etc. should be immediately supine, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved.