Neutral inflammatory cell phagocytosis assay

Neutrophils are an important part of the body's immune system, which can directly phagocytose and kill a variety of pathogenic microorganisms, antigen-antibody complexes. This test is mainly used to diagnose neutrophil phagocytic dysfunction. Basic Information Specialist classification: cardiovascular examination classification: immune examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: It indicates that the body is swallowed and the bactericidal function is defective. Normal value: Phagocytosis rate (n=50): 85.27%-91.04% Sterilization rate (n=50): 25.89%-40.55% Above normal: no information yet. negative: Positive: Tips: Actively cooperate with the doctor during the examination. Normal value Phagocytosis rate (n=50); (91.04±5.77)%. Sterilization rate (n=50) (32.72±7.83)%. It is best to investigate on your own according to the conditions of this laboratory. Clinical significance Defects in phagocytosis and bactericidal function, common in chronic granuloma, actin dysfunction, membrane glycoprotein deficiency, G6PD high defect, Chediak-Higashi syndrome. Defects in phagocytosis and bactericidal activity of Candida albicans are also seen in patients with more severe Candida albicans infections and malignancies. Low results may be diseases: phagocytic deficiency, chronic granulomatosis, primary immunodeficiency in children In order to achieve better comparability and reproducibility of the results, white blood cell concentration, bacterial concentration, reaction time and conditions should be standardized. Inspection process (1) Take 0.5 ml of white blood cell suspension and 0.5 ml of Candida albicans. After thoroughly mixing, the test tube was stoppered and incubated at 37 ° C for 45 min. (2) Centrifuge at 2000r/min for 10min after removal, discard the supernatant (retain a little), mix and drop the tablets, add 1 drop of the methylene blue solution and mix well. Cover the coverslip and check the oil after 5 minutes. Not suitable for the crowd People with reduced hematopoietic function such as leukemia, various anemia, myelodysplastic syndrome, or people with thrombocytopenia should pay attention to blood draw, and should not take more or more blood. Adverse reactions and risks 1. After the blood is drawn, do not press the needle hole to avoid subcutaneous hematoma. If there is a small piece of bruise in the blood, it is slightly tender, please don't panic, you can do hot compress after 24 hours to promote the absorption of blood. The general small amount of congestion will gradually absorb in 3 to 5 days and the color will become lighter and return to normal. 2. After the blood draw, symptoms such as dizziness, vertigo, fatigue, etc. should be immediately supine, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved.