Neutrophil Bactericidal Assay

When neutrophils phagocytose bacteria, the bacteria are killed by a combination of various factors in neutrophils (such as peroxidase, complement, etc.). In the absence of an enzyme (peroxidase), although the phagocytic function is normal, the phagocytic bacteria cannot be killed, and even the bacteria can be carried along with them to spread the infection. Basic Information Specialist classification: cardiovascular examination classification: immune examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: It shows that the body is less resistant to infection and susceptible to bacterial infection. Normal value: Sterilization rate: 25.89-40.55 Above normal: no information yet. negative: Positive: Tips: Pay attention to normal eating habits and pay attention to personal hygiene. Normal value The sterilization rate was 32.72±7.83. Clinical significance The bactericidal activity is reduced, indicating that the body is less resistant to infection and susceptible to bacterial infection. More common in congenital chronic granulomatosis and neutrophil dysfunction, diabetes, multiple myeloma, macroglobulinemia, chronic myeloid leukemia, myeloid and lymphoproliferative diseases, osteomyelitis, certain viruses Infection, rheumatoid arthritis, lupus erythematosus, long-term use of steroids, colchicine and vincristine, alcoholism and smokers. Low results may be diseases: macroglobulinemia, chronic granulomatosis, rheumatoid arthritis in the elderly (1) If intracellular bactericidal force measurement is required, the above-mentioned centrifuged cells which have not been cultured and cultured for 2 hours are respectively taken, and 10 ml of sterile distilled water is added to completely destroy the white blood cells under hypotonicity, and the solution is diluted with PBS. The sample was examined by the pour culture method to detect the number of colonies grown, and the ability of sterilizing in the leukocytes was inferred. (2) Because this test requires extracellular factors (antibody and complement) and intracellular factors, this test is suitable for examining phagocytic dysfunction or checking for the lack of complement and specific antibodies. Inspection process (1) The leukocytes of the subject were separated according to the natural sedimentation method, and the cells were mixed with 1.0 g/L gelatin in Tc199 to prepare a 5×106/ml cell suspension. (2) Take 0.5 ml of the white blood cell suspension, add 0.1 ml of Staphylococcus aureus suspension, and 0.4 ml of healthy human fresh serum (as opsonin). (3) Each specimen is in duplicate. One part was transplanted at 37 ° C for 2 h, centrifuged (1500 r / min) for 10 min; the other part was directly centrifuged without culture. (4) Each supernatant was diluted with PBS, and 1 ml of the sample was taken, 9 ml of ordinary agar medium was added, and the plate was poured, and cultured at 37 ° C for 24 hours, and the number of colonies was counted. Not suitable for the crowd People with reduced hematopoietic function such as leukemia, various anemia, myelodysplastic syndrome, or people with thrombocytopenia should pay attention to blood draw, and should not take more or more blood. Adverse reactions and risks 1. After the blood is drawn, do not press the needle hole to avoid subcutaneous hematoma. If there is a small piece of bruise in the blood, it is slightly tender, please don't panic, you can do hot compress after 24 hours to promote the absorption of blood. The general small amount of congestion will gradually absorb in 3 to 5 days and the color will become lighter and return to normal. 2. After the blood draw, symptoms such as dizziness, vertigo, fatigue, etc. should be immediately supine, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved.