Hepatitis B virus surface antibody (HBsAb)

Hepatitis B virus surface antibody (HBsAb) is an immunoreactive antibody (protective antibody) produced by hepatitis B surface antigen protein after a patient has been infected with hepatitis B virus. Hepatitis B virus surface antibody (HBsAb) appears 6-23 weeks after the initial infection of hepatitis B. It can last for several years. HBsAb positive indicates that it has been immunized. In normal population of China, the positive rate of HBsAb can reach 40%. Basic Information Specialist classification: Infectious disease examination and classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: It indicates that he has been infected with hepatitis B or has been vaccinated against hepatitis B. He has already developed immunity. Tips: If the examiner wants to check liver function (hepatitis B ultrasound) at the same time, it must be fasted. Normal value Qualitative negative. Clinical significance Positive is more common in people who have previously been infected with hepatitis B virus, and after injection of hepatitis B vaccine or hepatitis B surface antibody immunoglobulin. It is suggested that the hepatitis B virus has been immunized. However, it should be noted that for the above cases, it is necessary to determine the quantitative titer P/V value of HBsAb. When the titer is above 10, it indicates that the produced immunity can prevent hepatitis B, but when the effect value is below 10, the protective force is weakened. It is not even possible to prevent hepatitis B. Positive result may be disease: hepatitis B precautions The results must be analyzed along with other hepatitis B checkups. Inspection process (1) Use a carbonate buffer for proper dilution of anti-HBs·IgG (with anti-HBs serum or with a kit) to coat the polystyrene reaction plate, add 0.1ml per well (the last hole is not added) , as a blank). Cover and put at 4 ° C for 24 h. The next day, the cells were washed three times with Tris-HCl-Tween 20 buffer, and finally the plate was placed on a blotting paper to allow the liquid to flow out. (2) Add 0.1 ml of the serum to be tested to each well (observe the titer for different dilution). Positive and negative serum controls were performed simultaneously on each plate. After capping, the solution was removed at 37 ° C for 2 h, and washed three times as above. (3) Add the substrate solution (0.1mol/L Na2HPO45.14ml, 0.05mol/L citric acid 4.86ml, o-phenylenediamine 4mg, 3% H2O20.05ml, protected from light, ready for use) 0.1ml. Dark in room temperature for 15 to 30 minutes. After the positive control wells were fully developed, the reaction was stopped by adding 2 mol/L H2SO4 0.05 ml to each well. Not suitable for the crowd People with reduced hematopoietic function such as leukemia, various anemia, myelodysplastic syndrome, or people with thrombocytopenia should pay attention to blood draw, and should not take more or more blood. Adverse reactions and risks 1. After the blood is drawn, do not press the needle hole to avoid subcutaneous hematoma. If there is a small piece of bruise in the blood, it is slightly tender, please don't panic, you can do hot compress after 24 hours to promote the absorption of blood. The general small amount of congestion will gradually absorb in 3 to 5 days and the color will become lighter and return to normal. 2. After the blood draw, symptoms such as dizziness, vertigo, fatigue, etc. should be immediately supine, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved.