Blood Mycoplasma Antibody Assay

Mycoplasma antibodies are used for the diagnosis and differential diagnosis of mycoplasma infectious diseases. In the complement fixation test, indirect hemagglutination test, and enzyme-linked immunosorbent assay, the serum antibody was increased by more than 4 times during the recovery period, and the titer was above 132. Basic Information Specialist classification: Infectious disease examination and classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Infected with mycoplasma infectious diseases. Tips: Can be used for complement binding test, indirect hemagglutination test, enzyme-linked immunosorbent assay, indirect immunofluorescence test, detection of Mycoplasma pneumoniae membrane protein antibody, etc., simultaneous detection by different methods can increase the positive rate. Normal value negative. Clinical significance This test is adapted to the etiology of pneumonia. 1. In the complement fixation test, indirect hemagglutination test, enzyme-linked immunosorbent assay, the serum antibody was increased by more than 4 times during the recovery period, and the titer was above 132. 2. Condensate set test The recovery period of serum complement-binding antibody titer is greater than or equal to 164, or the increase of two or more serum titers by more than 4 times may consider mycoplasma infection. 3. The slight increase in titer can be found in influenza, cirrhosis, and infectious mononucleosis. Positive result may be disease: Precautions for mycoplasma infection 1. Can be tested by complement binding test, indirect hemagglutination test, enzyme-linked immunosorbent assay, indirect immunofluorescence test, detection of Mycoplasma pneumoniae membrane protein antibody, etc. Simultaneous detection by different methods can increase the positive rate. 2. The antibody detected by the complement-binding assay is mainly IgM. The antibody generally rises at 1 week of onset, reaches a peak at 1 month, and then gradually decreases, and the positive rate at re-infection is low. 3. Indirect hemagglutination test mainly detects serum Mycoplasma pneumoniae IgM. The antibody appears to occur around 1 week, peaks at 2-3 weeks, decreases after 2-3 months, and sometimes lasts for 6 months to 1 year. This method is slightly more sensitive than the complement binding test method. 4. The enzyme-linked immunosorbent assay uses M. pneumoniae as an antigen to examine IgM and IgG, respectively. This method has high sensitivity but poor specificity. 5. The indirect immunofluorescence test uses the Mycoplasma pneumoniae colony print as an antigen, and the indirect immunofluorescence assay has strong specificity for detecting Mycoplasma pneumoniae IgM and IgG. Inspection process 1. Take 10 small test tubes, respectively, mark 1-10, add 0.2ml of normal saline to each tube, use the graduated pipette to absorb 0.2ml of the test subject's serum, add to the first tube, mix well, and then use the same pipette from the first test tube. Aspirate 0.2 ml into the second tube, mix well, then pipet 0.2 ml into the third tube and dilute it. Take 0.2 ml of the 10th tube and discard it. Make 1:2, 1:4... 2. Then add an equal amount of 5% red blood cell suspension containing the corresponding antigen to each tube, shake the tube rack to mix it, place it in a 37 ° C water bath for 1 h, centrifuge at 3000 r / min for 15 s, observe the agglutination results and record; For those with agglutination intensity of "1+" or less and non-aggregation, wash with physiological saline for 3 times, and finally blot the supernatant; add 2 drops of physiological saline, and take 2 drops of anti-human globulin reagent per tube to 3000r/ Centrifuge for 15 s, observe the agglutination results and record. The reciprocal of the highest dilution at which agglutination occurs is the titer. Not suitable for the crowd There are no special taboos. Adverse reactions and risks There are no related complications and hazards.