serum total complement

The total complement activity is based on complement to enable hemolysis of sheep red blood cells sensitized against anti-govine erythrocyte antibody (hemolysin), and its degree of hemolysis is related to total complement activity, but not linear. The total complement activity assay primarily reflects the activity of complement (C1-C9) after activation by conventional pathways. Changes in serum total complement activity have an extremely important role in the diagnosis and treatment of certain diseases. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: The serum to be tested must be fresh. If left at room temperature for more than 2 hours, the complement activity will decrease. Normal value The normal value of healthy people measured by this method is 50-100 U/ml. Clinical significance In many pathological conditions, serum complement levels can be changed, so the clinical changes in the content of complement, such as total complement activity, changes in the individual components of complement, especially C3 and C4, etc., for the diagnosis of certain diseases, etiology and Prognosis has a certain meaning. However, the decrease in the amount of complement is not necessarily an immune disorder or an immune disease. It is known that ischemia, coagulative necrosis and toxic necrosis can release more proteolytic enzymes in tissues, leading to complement hemolytic activity and a decrease in each complement component. Some congenital complement deficiency can also cause a defect in a complement component, so a detailed analysis of the specific situation is required. Precautions (1) The serum to be tested must be fresh. If left at room temperature for more than 2 hours, the complement activity will decrease. (2) The hemolytic activity of complement is affected by many factors, such as sheep red blood cell concentration and the amount of sensitized antibodies. When the antibody molecule adsorbed per uniform sensitive red blood cell is less than 100, the degree of red blood cell solubility decreases as the cell concentration increases. When sensitized with a high concentration of antibodies, the degree of hemolysis increases as the cells increase. A doubling of the red blood cell concentration can increase the amount of 50% hemolysis complement by about 25%. The presence of calcium and magnesium ions stabilizes the hemolysis system. However, when the content is too much, the hemolysis reaction is inhibited. Therefore, in the test, it is necessary to strictly control all aspects of the reaction. (3) This test cannot measure the absolute value of the complement protein, nor can it measure the components in the properdin system. Inspection process (1) The serum to be tested is diluted 1:20, and 0.2 ml of the serum to be tested is aspirated into a test tube, and 3.8 ml of barbital buffer is added and mixed. (2) Take 10 tubes and mix with liquid. (3) First compare the above tubes with the 50% hemolysis standard tube, select two tubes close to the standard tube, and read with a spectrophotometer at a wavelength of 542 nm to find a closer to the standard optical density. One tube. The total complement value was determined according to the standard based on the amount of diluted serum added to the tube. Not suitable for the crowd There are no special taboos. Adverse reactions and risks No relevant records.