Determination of serum complement C4 content

C4 is one of the 11 complement components in the blood. Determination of C4 content contributes to the diagnosis and treatment of autoimmune diseases such as systemic lupus erythematosus (SLE). Increased: seen in the acute phase of rheumatic fever, nodular arteritis, dermatomyositis, myocardial infarction, liver cancer and various types of polyarthritis. Reduction: seen in systemic lupus erythematosus, chronic active hepatitis, multiple sclerosis encephalitis, IgA nephropathy, advanced pancreatic cancer. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. Normal value 0.12 ~ 0.36 g / liter (12 ~ 36 mg / dl). (Note the specific reference value depends on each laboratory.) Clinical significance Increased in acute rheumatic fever, nodular arteritis, dermatomyositis, myocardial infarction, liver cancer and various types of polyarthritis. Reduction in systemic red increase is seen in the acute phase of rheumatic fever, nodular arteritis, dermatomyositis, myocardial infarction, liver cancer and various types of polyarthritis. Reduced in systemic lupus erythematosus, chronic active hepatitis, multiple sclerosis encephalitis, IgA nephropathy, advanced pancreatic cancer. Lupus, chronic active hepatitis, multiple sclerosis encephalitis, IgA nephropathy, advanced pancreatic cancer. Precautions First, the precautions before blood draw 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the physical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. If you have a history of fainting blood, please explain in advance and make special arrangements. Second, should pay attention after blood draw 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of dizziness, such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of sugar water, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Third, venous blood 2ml, no anticoagulation, separation of serum as soon as possible. Inspection process (1) Preparation of antibody agarose plate: The melted 10.0 g/L agarose gel was mixed with an appropriate concentration of anti-C4 serum, and poured (8 cm × 8.5 cm), and the cells were perforated after cooling and solidification. (2) 8 holes were punched at 1 cm from one side, the hole diameter was 3 mm, the hole pitch was 5 mm, and the agarose in the hole was picked. (3) A 1:4 dilution of the test serum and 10 μl of different concentrations of C4 reference serum were added to each well. The three-layer filter paper was used as a bridge, and electrophoresis was carried out in an electrophoresis tank of 0.025 mol/L pH 8.6 barbital buffer. One end of the hole was connected to the cathode, the terminal voltage was 3 to 4 V/cm, and after electrophoresis for 4 to 5 hours, the power was turned off. , take off the agar plate measurement. Not suitable for the crowd There are no special taboos. Adverse reactions and risks No related complications and hazards.