Serum complement C1q

C1q is an important component of complement C1, with a molecular weight of 390,000. It consists of six identical subunits that form a symmetric hexamer. When two or more C1qs bind to the Fc segment of IgM or IgG in an immune complex, C1q Changes in conformation result in sequential activation of C1r and C1s, triggering the classical activation pathway of complement. Clinically, one-way immunodiffusion method is commonly used to determine C1q. Basic Information Specialist classification: inspection classification: immune examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Reduced in: active mixed connective tissue disease. Normal value: One-way immunodiffusion method: 15.7-23.7 mg / dl Above normal: Increased in: osteomyelitis, rheumatoid arthritis, systemic lupus erythematosus (SLE), vasculitis, scleroderma, gout, active allergic purpura. negative: Positive: Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. Normal value The one-way immunodiffusion method was 0.197 ± 0.04 g / liter (19.7 ± 4.0 mg / dl). Clinical significance Increased in: osteomyelitis, rheumatoid arthritis, systemic lupus erythematosus (SLE), vasculitis, scleroderma, gout, active allergic purpura. Reduced in: active mixed connective tissue disease. High results may be diseases: vasculitis, scleroderma, rheumatoid arthritis, gout, osteomyelitis precautions First, the precautions before blood draw 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the physical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. If you have a history of fainting blood, please explain in advance and make special arrangements. Second, should pay attention after blood draw 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of dizziness, such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of sugar water, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Third, venous blood 2ml, no anticoagulation, separation of serum as soon as possible. If it is not possible to measure it in time, note that the serum is stored at -20 degrees Celsius. Inspection process Anti-C1q serum was diluted to a total amount of 4 ml with 0.1 g/L thiomersal physiological saline at a potency, pre-warmed in a water bath at 56 ° C, and mixed with 20.0 g/L agarose (24.0 g/L agar) which was melted and cooled to 56 ° C. uniform. A 6 cm × 8 cm plate was poured, and the wells were perforated with 10 μl of the serum to be diffused for 48 hours in a 37 ° C wet room. The rest of the procedure was determined by immunoglobulin single diffusion method. Standard curve preparation A standard curve was prepared by diluting a reference standard serum of known C1q content 1:2, 1:3, 1:4, 1:6, 1:8 (specifically determined by immunoglobulin single diffusion method). Not suitable for the crowd There is no inappropriate crowd. Adverse reactions and risks 1, after blood draw may appear symptoms of dizziness such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of sugar water, until the symptoms are relieved before physical examination. 2. If there is localized congestion, use a warm towel after 24 hours to promote absorption.