serum interleukin 8

IL-8 is a low molecular weight (6-8 kD) polypeptide. This cell factor can be produced by various cells such as monocytes, lymphocytes, fibroblasts, and endothelial cells stimulated by LPS, TNF or IL-1. . The characteristic biological effect of IL-8 is chemotaxis and activation of neutrophils and thus involvement in neutrophil-mediated tissue damage. In many chronic inflammatory diseases, such as rheumatoid arthritis, psoriasis, local or serum IL-8 abnormalities, quantitative detection of IL-8 is important for the study of the occurrence, development and guidance of these diseases. Basic Information Specialist classification: inspection classification: immune examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. Fasting for 12 hours before taking blood, taking fresh blood for examination. Normal value ELISA 8.1 ~ 21.3 μg / L. Clinical significance Elevation: rheumatoid arthritis, nephrotic syndrome, hemorrhagic fever. High results may be diseases: renal damage of rheumatoid arthritis, epidemic hemorrhagic fever, rheumatoid arthritis in the elderly, nephrotic syndrome The method is specific and sensitive. The minimum detection amount is about 100pg/ml, and the repeatability is good. The key is to prepare rabbit anti-IL-8 IgG with high titer and high purity. Inspection process (1) Rabbit anti-human IL-8 IgG (1 μg/ml) was coated, diluted with a carbonate buffer, and added to each well of the plate, 50 μl/well. 4 ° C overnight. (2) The plate was washed 3 times with a washing solution (PBS-0.05% Tween 20), and a blocking solution (PBS containing 2% bovine serum albumin) was added at 200 μl/well for 2 hours at 37 °C. (3) Add the sample to be tested and the serially diluted rIL-8 standard, 50 μl/well. All samples and each dilution standard are duplicate or three wells, 37 ° C for 1 h. (4) Plate was washed 3 times, biotinylated rabbit anti-IL-8 IgG (35 μg/ml) 50 μl/well was added, and incubated at 37 ° C for 30 min. (5) The plate was washed 3 times, and a 1:5000 dilution of avidin-HRP was added, 100 μl/well, and incubated at 37 ° C for 30 min. (6) The plate was washed 3 times, 100 μl/well of the substrate solution was added, and color development was carried out for 5 min at room temperature, and 50 μl of 2 mol/L H 2 SO 4 was added to each well to terminate the reaction. (7) A490nm was measured by a microplate reader. Not suitable for the crowd There is no inappropriate crowd. Adverse reactions and risks It is a safe check and is harmless to the body.