Heterophilic agglutination absorption test

There is a heterophilic antigen (Forssmanantigen, F.antigen) between humans, animals, plants and microorganisms, which can stimulate the body to produce a heterosexual antibody (Fsantigen lectin) (Fsantibody lectin) (F.antibody). In addition to reacting with the corresponding antigen, it can also react with red blood cells or tissue infusions of other animals. Detection of F-antibody in the patient's serum can assist in the clinical diagnosis and treatment of certain diseases. Basic Information Specialist classification: Infectious disease examination and classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: (1) Normal human serum heterophilic antibody titer is generally 1:160 positive. (2) Heterophilic antibodies in normal human serum are generally not absorbed by bovine red blood cells, but can be completely absorbed by guinea pig kidney tissue. Positive: (l) Heterophilic antibodies in the serum of patients with infectious mononucleosis can be completely absorbed by bovine red blood cells and partially absorbed by the kidney tissues of guinea pigs. (2) The anti-serum (horse serum) or the heterophilic antibody in the serum of the serum patients can be completely absorbed by the guinea pig kidney tissue and bovine red blood cells. (3) Heterologous antibodies in patients with infectious mononucleosis are not always positive, first appeared on the 4th day of onset, most occurred after the first week, often lasted until the third week after onset, a small number of patients Can last for several months. It has been reported that the number of cases with meaningful antibody titers in the first week of the disease is about 40%, and it rises to 60% in the second week. However, due to the inconsistent timing of heterophilic antibodies, it is necessary to review them regularly, such as an increase in antibody titer during the course of the disease. Tips: Please cooperate with the doctor when checking. Normal value (1) Normal human serum heterophilic antibody titer is generally <1:80, 1:80 to 1:160 is suspicious, and >1:160 is positive. (2) Heterophilic antibodies in normal human serum are generally not absorbed by bovine red blood cells, but can be completely absorbed by guinea pig kidney tissue. Clinical significance (l) Heterophilic antibodies in the serum of patients with infectious mononucleosis can be completely absorbed by bovine red blood cells and partially absorbed by the kidney tissues of guinea pigs. (2) The anti-serum (horse serum) or the heterophilic antibody in the serum of the serum patients can be completely absorbed by the guinea pig kidney tissue and bovine red blood cells. (3) Heterologous antibodies in patients with infectious mononucleosis are not always positive, first appeared on the 4th day of onset, most occurred after the first week, often lasted until the third week after onset, a small number of patients Can last for several months. It has been reported that the number of cases with meaningful antibody titers in the first week of the disease is about 40%, and it rises to 60% in the second week. However, due to the inconsistent timing of heterophilic antibodies, it is necessary to review them regularly, such as an increase in antibody titer during the course of the disease. Positive results may be diseases: infectious mononucleosis, pediatric infectious mononucleosis, extra-chondral sarcoma, small intestinal leiomyosarcoma A small number of patients with lymphoblastic cell carcinoma, monocytic leukemia, lymphosarcoma or tuberculosis may also have mild positive results. Inspection process (1) Preliminary heterophilic agglutination test: 1 Arrange 10 tubes, add 1 ml of normal saline to the first tube, and 0.5 ml of the remaining tubes. 2 The first tube was added with 0.2 ml of the test serum, and after mixing, 0.5 ml was added to the second tube, mixed, and sequentially diluted to the 9th tube, and the 10th tube was used as a control. 3 Add 1% sheep red blood cell suspension 0.5 ml to each tube. At this time, the serum dilution ratio of 1-9 tube is 1:10, 1:20, 1:40, 1:80, 1:160, 1:320,1 640, 1:1280, 1:2560. 4 Mix, 37 ° C water bath for 1 h, if there is no agglutination, it should be placed at 4 ° C overnight, and then placed in a 37 ° C water bath for 1 h, for the final observation. 5 The result is determined by the same test tube hemagglutination test, the highest dilution tube of the serum appears (++) agglutination, and the reciprocal of the serum dilution factor of the tube is the titer of the serum. (2) Identification of heterophilic agglutination test: 1 Guinea pig kidney tissue absorption test Take 1% of 20% guinea pig kidney tissue suspension, add 0.2ml of test serum, and mix. The cells were allowed to stand for 1 h at room temperature, and shaken frequently; centrifuged at 1500 r/min, and the supernatant was taken as a 1:5 diluted absorbed serum; the serum was retested for antibody titer according to preliminary heterophilic agglutination test. The result is judged as before. 2 bovine red blood cell absorption test Take 20% bovine red blood cell suspension 1ml, add 0.2ml of test serum, mix, let stand for 1h at room temperature, often shaken during the period. Centrifuge at 1500 r/min; for 10 min, the supernatant was a 1:5 diluted absorbed serum. The serum was retested for antibody titer according to a preliminary heterophilic agglutination test. The results are judged as described above. Not suitable for the crowd no. Adverse reactions and risks Nothing.