Treponema pallidum gelatin agglutination test

The syphilis spiral concise gelation test uses ultrasound-cleaved treponema pallidum as an antigen, and the sensitized red gelatin granules bind to treponema pallidum antibodies in human serum or plasma to produce a macroscopic red agglutination reaction. Basic Information Specialist classification: Infectious disease examination and classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Syphilis infection. Tips: Pay attention to normal eating habits and pay attention to personal hygiene. Unclean sexual intercourse is prohibited. Normal value The reaction was negative. Clinical significance 1. The TPPA test has high sensitivity and strong specificity, and is a confirmatory test for the diagnosis of syphilis. 2. Patients with positive TPPA test, because of the strong ability of memory cell antibody to replicate, the sensitivity of specificity test is high, even if it is treated with anti-syphilis, it is life-positive, so it can not be used as an indicator for observation of therapeutic effect. However, according to clinical observations, serum of patients with early syphilis such as TPPA test is a weak positive reaction, which may turn negative over time. 3. The specificity test for the first-stage syphilis can occur one week earlier than the non-specific test. The first occurrence of syphilis is the IgM body, which can be detected from the serum in the second week after infection, while the IgG antibody needs to be detected in the fourth week after infection. 4. The syphilis confirmation test, if the general qualitative test is positive, can be used as a diagnostic basis for syphilis and quantitative tests if necessary. The specific antibody titer can be reduced to varying degrees after effective treatment, but the chance of turning negative is very small. Such tests are highly specific and rarely have false positives. However, according to statistics, there can also be 1% of biological false positives. Abnormal results: 1 stage syphilis. That is, hard chancre, with an incubation period of 2 to 4 weeks, a dark red hard mass, a shallow ulcer, a cartilage-like hardness, and a swollen peripheral lymph node. 2 second phase syphilis. After 1 to 2 months of primary syphilis, sympathetic rash, rash, pimples, pustular rash, etc. occur in the skin and mucous membranes of the whole body. Mucosal plaques, flat wet phlegm can occur in the mucosa, and the infection is strong. 3 third-phase syphilis. Occurred 2 to 3 years or even 10 years after infection, the skin is a gum-like swelling, and may also involve bone, joint, heart, blood vessels, manifesting aortic inflammation, aortic insufficiency and aortic aneurysm, etc., invading the nerve Spinal cord paralysis, general paralysis (paralytic dementia) and so on. The person in need of examination has the above symptoms. Positive results may be diseases: Phase I syphilis, syphilis precautions 1. The TPHA and TPPA reaction plates should be clean and free of foreign matter in the holes to avoid false positives. To use a U-shaped plate, you cannot use a V-shaped plate. 2. The kit should not be set below 0 °C to prevent freezing. The different batches of reagents should not be mixed. 3. The test temperature is suitable for 15 °C ~ 25 °C. When it is hot, the wet box should be placed in the air-conditioned room. 4. If there is no agglutination reaction in the sensitized cells, a reabsorption test should be performed or other diagnostic test methods should be used. Inappropriate crowd: No. Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Unclean sexual intercourse is prohibited. Requirements for inspection: Actively cooperate with the doctor. Inspection process material: 1. Dissolution solution A: for dissolving sensitized particles C and non-sensitized particles D. 2. Serum Dilution B: For dilution of serum samples. 3. Sensitized particles C. 4. Non-sensitized particles D. 5. Control positive serum E. 6. U-shaped reaction plate. method: (1) 100 μl of the B solution was added to the 8th hole, and 25 μl of the B solution was added to the 1st, 2nd, 3rd, 4th, 5th, and 6th holes, respectively. (2) Serum dilution: Pipette 25 μl of the serum to be tested into the 8th hole, mix and take 25 μl to the first well, repeat the dilution step to the 6th well, mix and discard 25 μl of the liquid. (3) Adding sensitized and unsensitized particles: mixing the cell working solution, adding 25 μl of unsensitized particles to the second well; adding 25 μl of sensitized particles to the third, fourth, fifth and sixth wells; The shaker was shaken for 30 s, placed in a humid chamber, and incubated at 15 ° C to 25 ° C for 2 h to observe the results. result: Positive 2+~4+: Particle agglutination covers the entire bottom of the hole, with a polygonal membrane and rough edges. Positive 1+: The agglutination of the particles is a polygonal rough ring. Suspicious ±: The particle concentration is smooth and small in the shape of a ring. Negative -: The particles gather in the center of the bottom of the hole, with a bright, smooth edge. The quantitative test uses the highest dilution of the positive reaction (1+) as the final titer. Not suitable for the crowd There are no special taboos. Adverse reactions and risks There are no related complications and hazards.