Treponema protein complement fixation assay

The spirochete complement binding assay uses an antigen-antibody complex to bind to complement, and a test containing a known concentration of complement in a complement reaction solution to reduce the concentration to detect a Treponema pallidum antigen or antibody. Basic Information Specialist classification: Infectious disease examination and classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Positive syphilitic cardiovascular disease. Tips: Blood collection is recommended for fasting. Normal value negative. Clinical significance Abnormal result Positive syphilitic cardiovascular disease. Need to check the crowd: The skin is sluggish and pale, wrinkles such as the appearance of the elderly, slow weight gain, low crying and hoarseness, often accompanied by hypothermia, anemia, hepatosplenomegaly, lymphadenopathy and alopecia. Precautions Before the test: It is recommended to fasting. At the time of examination: venous blood collection, no anticoagulation. Inspection process The test consisted of two phases: first, the antiserum that inactivated the complement after being treated at 56 ° C for 30 minutes was mixed with the antigen and complement (usually diluted with guinea pig serum) to react. The second is to add sheep red blood cells (sensitized red blood cells) that have been combined with anti-sheep erythrocyte antibodies. When sufficient antigen-antibody reaction was established in the initial stage of consumption of complement, no hemolysis of sensitized red blood cells occurred, but the remaining complement caused a hemolysis reaction. The syphilis complement binding reaction (Wassermannreaction) used to examine syphilis is the most commonly performed complement binding assay. Not suitable for the crowd Not suitable for the crowd: people who are hemolyzed and fat. Adverse reactions and risks There are no related complications and hazards.