rapid plasma reagin test

The rapid plasma responsiveness test is an improvement of the VDRL antigen, and its sensitivity and specificity are similar to those of VDRL. The advantage is that the results can be read by the naked eye. The cardiolipin is used as an antigen to measure anti-cardiolipin antibodies in serum, also known as responsive factors. The test is highly sensitive and low in specificity, and is prone to biological false positives. After adequate treatment of patients with early syphilis, the responsiveness can disappear, and the early untreated patients can be reduced or disappeared in some patients. Currently, screening and quantitative tests are generally used to observe efficacy, recurrence and reinfection. Basic Information Specialist classification: Infectious disease examination and classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Mainly found in syphilis, the positive rate can reach 80% to 95%. Tips: Pay attention to normal eating habits and pay attention to personal hygiene. Normal value The reaction was negative. Clinical significance Non-treponema pallidum antigen test, one of the commonly used methods for syphilis. Abnormal results syphilis has different abnormal results in different periods. 1 stage syphilis. That is, hard chancre, with an incubation period of 2 to 4 weeks, a dark red hard mass, a shallow ulcer, a cartilage-like hardness, and a swollen peripheral lymph node. 2 second phase syphilis. After 1 to 2 months of primary syphilis, sympathetic rash, rash, pimples, pustular rash, etc. occur in the skin and mucous membranes of the whole body. Mucosal plaques, flat wet phlegm can occur in the mucosa, and the infection is strong. 3 third-phase syphilis. Occurred 2 to 3 years or even 10 years after infection, the skin is a gum-like swelling, and may also involve bone, joint, heart, blood vessels, manifesting aortic inflammation, aortic insufficiency and aortic aneurysm, etc., invading the nerve Spinal cord paralysis, generalized paralysis (paralytic dementia). Congenital syphilis has early congenital syphilis, equivalent to the second day of the day, but heavier. Late congenital syphilis is similar to the acquired day, but few heart, blood vessel and neuropathy occur. Mainly for substantial keratitis, neurological deafness, Hutchinson's teeth (central incisors in the upper incisors, small upper and upper, equal width and width), sabre-shaped humerus and so on. There may be latent syphilis between stages, asymptomatic, and only seropositive. The treatment uses penicillin or erythromycin, tetracycline, and the like. The person in need of examination has the above symptoms. Positive results may be diseases: syphilitic keratopathy, third-stage syphilis, syphilitic uveitis considerations (1) The test shall be carried out at 23 to 29 °C. (2) Different batches of reagent components must not be mixed. (3) The kit should be treated as containing infectious materials. (4) RPR test is a serum serotonin test, which may have biological false positives. It should be diagnosed after clinical history or verification with treponema pallidum specific antibody test. Inappropriate crowd: temporarily unknown. Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Prohibition of unclean sexual intercourse. Requirements for inspection: Actively cooperate with the doctor. Inspection process Qualitative test: (1) 0.05ml (without inactivation) of serum or plasma to be tested is placed in the test collar and spread evenly. (2) Add 1 drop of RPR reagent to the serum in the circle. (3) Shake for 8 minutes at 100 rpm and observe the results with the naked eye. Potency measurement: The serum to be tested was double-diluted, and the test method and results were judged as the same qualitative test. The result is judged: Positive reaction (+++~++++): medium and large clots, clear solution (R). Weak positive reaction (+~++): Small particle aggregates (W) are visible. Suspicious reaction (±): Small roughness is visible and the distribution is irregular. Negative reaction (-): The antigen particles were evenly distributed without a mass (N). Not suitable for the crowd There are no special taboos. Adverse reactions and risks There are no related complications and hazards.