Rapid plasma reagin ring card test

The rapid plasma reaxin loop card assay is a non-specific reaction to serological detection of syphilis. It is used to detect the presence or absence of anti-lipid antigens in the serum of patients, and is often used in screening and epidemiological investigation of syphilis patients. The rapid plasma-reactive cyclic card experiment is a semi-quantitative test that is fast, simple, and does not require a microscope, and is suitable for a large number of screening experiments. Basic Information Specialist classification: Infectious disease examination and classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Rapid plasma responsive ring card experiment. Tips: Prohibition of unclean sexual intercourse is prohibited. Normal value The reaction was negative. Clinical significance The antigen used in the rapid plasmin circular card experiment was a lipidoid antigen adsorbed on carbon particles, mixed with one drop of serum in the circle of the RPR card, and positive if agglomerated particles appeared. Abnormal results: 1 stage syphilis. That is, hard chancre, with an incubation period of 2 to 4 weeks, a dark red hard mass, a shallow ulcer, a cartilage-like hardness, and a swollen peripheral lymph node. 2 second phase syphilis. After 1 to 2 months of primary syphilis, sympathetic rash, rash, pimples, pustular rash, etc. occur in the skin and mucous membranes of the whole body. Mucosal plaques, flat wet phlegm can occur in the mucosa, and the infection is strong. 3 third-phase syphilis. Occurred 2 to 3 years or even 10 years after infection, the skin is a gum-like swelling, and may also involve bone, joint, heart, blood vessels, manifesting aortic inflammation, aortic insufficiency and aortic aneurysm, etc., invading the nerve Spinal cord paralysis, generalized paralysis (paralytic dementia). Congenital syphilis has early congenital syphilis, equivalent to the second day of the day, but heavier. Late congenital syphilis is similar to the acquired day, but few heart, blood vessel and neuropathy occur. Mainly for substantial keratitis, neurological deafness, Hutchinson's teeth (central incisors in the upper incisors, small upper and upper, equal width and width), sabre-shaped humerus and so on. There may be latent syphilis between stages, asymptomatic, and only seropositive. The treatment uses penicillin or erythromycin, tetracycline, and the like. The person in need of examination has the above symptoms. Precautions It is a non-specific syphilis serological test, which is often used for early diagnosis of syphilis, but is not sensitive to latent syphilis and neurosyphilis. TPHA detects specific treponema pallidum antibodies in serum with high sensitivity and specificity. Once the test is positive, whether it is treated or not, or whether the disease is active, it is usually positive for life. The change in titer is not related to the activity of syphilis. Therefore, it cannot be used as an indicator for evaluating efficacy or determining recurrence and reinfection. It can only be used as syphilis. Confirmation test. Inappropriate crowd: No. Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Unclean sexual intercourse is prohibited. Requirements for inspection: Actively cooperate with the doctor. Inspection process method: (1) Take 50ul of serum to be tested, add it to the circle of the card, and spread it into the entire circle. Both positive and negative controls were performed at the same time. (2) One drop of RPR antigen was added to each serum. (3) Rotate the card for 8 minutes at a speed of about 100 rpm, and immediately observe the result with the naked eye. As a result, it was judged that the RPR white paper card was observed, and no black carbon particles agglomerated in the reaction circle of the negative sample, and obvious black agglomerated carbon particles or flakes appeared in the reaction ring of the positive sample. In order to better distinguish between weak positive and negative results, the paper card is tilted to rotate at 30° to the horizontal, so that the serum and the antigen are rotated in a circle, and the result can be observed more clearly. According to the size of the particles or flocs, record + ∽++++ This result. Specimens with positive qualitative test, if necessary, can make 16 dilutions such as 1:2 - 1:32 on the RPR card, and then do semi-quantitative experiments according to the above qualitative test method. Not suitable for the crowd There are no special taboos. Adverse reactions and risks There are no related complications and hazards.