soluble interleukin-2 receptor

The soluble interleukin-2 receptor (SIL-2R) is a complex mucin with information on binding to anti-Tac monoclonal antibody and interleukin-2, and does not bind to interleukin-2. Requires any cofactor, soluble interleukin-2 receptor is an important immunosuppressive agent that neutralizes interleukin-2 around activated T cells, attenuates the endocrine effects of the body, and inhibits the cloning of activated T cells. Amplification. Soluble interleukin-2 receptor binds to interleukin-2 and is present in the blood, prolonging the half-life of interleukin-2 in vivo and transporting interleukin-2 to distant interleukin production sites. organization. The soluble interleukin-2 receptor releases interleukin-2 to bind to the high-affinity soluble interleukin-receptor, thereby providing positive and negative immunomodulatory effects. Basic Information Specialist classification: examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: no. Normal value: Normal value: 206-386U/mL Above normal: The level of soluble interleukin-2 in normal human serum is lower, and its abnormal changes are mainly manifested as increased. negative: Positive: Tips: Do not wear clothes that are too small or too tight, so as to avoid the sleeves being too tight when blood is drawn or the sleeves are too tight after blood drawing, causing blood vessels in the arms. Normal value The normal reference value for the determination of SIL-2R in serum by double antibody sandwich method was (296 ± 90) U/mL. Clinical significance Abnormal results The serum levels of soluble interleukin-2 receptors in normal humans are low, and the abnormal changes are mainly manifested in the increase. The examination of the changes in soluble interleukin-2 receptors can be used for the diagnosis and identification of certain diseases. Provide a basis for prognosis. (1) Cells with interleukin-2 receptor are found in the brain tissue of patients with soluble interleukin-2 receptor and multiple sclerosis multiple sclerosis. The concentration of soluble interleukin-2 receptor in the serum of patients with multiple sclerosis was significantly increased. In patients with multiple sclerosis, the serum interleukin-2 receptor concentration is high, while the stable phase is reduced. However, the relationship between the level of soluble interleukin-2 receptor in the cerebrospinal fluid of patients with multiple sclerosis and the diagnosis of multiple sclerosis, the severity of the disease, and the degree of activity of multiple sclerosis are unclear. (2) Soluble interleukin-2 receptor and leukemia and lymphoid malignant diseases In the above diseases, serum soluble interleukin-2 receptor is significantly increased, and the level of soluble interleukin-2 receptor is decreased when the condition is relieved. (3) Soluble interleukin-2 receptor and AIDS (AIDS) and AIDS-related syndrome (ARC) have elevated levels of soluble interleukin-2 receptor and soluble interleukin-2 receptor levels in this disease There was a negative correlation with the number of CD4 cells. (4) Soluble interleukin-2 receptor and organ transplant rejection At this time, serum soluble interleukin-2 receptor levels can dynamically respond to transplants and other autoimmune diseases such as active SLE, serum soluble leukocyte The receptor-2 receptor is significantly elevated. (5) Relationship between soluble interleukin-2 receptor and interleukin-2 in multiple sclerosis The expansion of antigen-activated T cell subsets in multiple sclerosis requires the synthesis and secretion of interleukin-2 with high solubility. Expression of the interleukin-2 receptor. Activated B cells, natural killer cells and monocytes have interleukin-2 receptor expression, and serum soluble interleukin-2 receptor and interleukin-2 are closely related and can be reacted to some extent. The degree of cellular immunity of a patient does not reflect the severity of the patient's clinical condition. Need to check the population suspected of having the above conditions. High results may be diseases: cirrhosis precautions Inappropriate people: generally no special population. Taboo before the examination: It is necessary to cooperate with the doctor to write the correct name, neat and tidy, to avoid confusion caused by the same name or similar names. With these in mind, blood draws are more convenient and faster, and you can better save yourself time for diagnosis. Requirements for inspection: Do not wear clothes that are too small or too tight in cuffs to avoid the sleeves being too tight when blood is drawn or the sleeves are too tight after blood drawing, causing blood vessels in the arms. Different laboratory items should be asked by the doctor and treated differently. Inspection process The double antibody sandwich method is the most commonly used method for detecting antigens. The steps are as follows: 1. Attach a specific antibody to a solid phase carrier to form a solid phase antibody: wash to remove unbound antibody and impurities. Second, add the test specimen: make it react with the solid phase antibody for a period of time, let the antigen in the specimen bind to the antibody on the solid phase carrier to form a solid phase antigen complex. Wash to remove other unbound material. 3. Enzyme-labeled antibody: The antigen on the solid phase immune complex is bound to the enzyme-labeled antibody. Unbound enzyme-labeled antibody was thoroughly washed. At this time, the amount of enzyme carried on the solid phase carrier is positively correlated with the amount of the substance to be tested in the specimen. 4. Addition of substrate: The enzyme-catalyzed substrate in the sandwich composite becomes a colored product. Qualitative or quantitative determination of the antigen is based on the extent of the color reaction. According to the same principle, the macromolecule antigen is separately prepared into a solid phase antigen and an enzyme-labeled antigen conjugate, and the antibody in the sample can be determined by a double antigen sandwich method. Not suitable for the crowd There are no special taboos. Adverse reactions and risks There are no related complications and hazards.