anti-histone antibody

Histone is the most abundant protein in the nucleus, and its complex with DNA is called chromatin. The most basic subunit structure of chromatin is the nucleosome, which is surrounded by 8 histone molecules by a DNA strand consisting of 146 base pairs (2 H2A-H2B heterodimers sandwiching 2 impurities) The dimer H2-H4) consists of two loops of the core and the extranuclear histone H1 and the junction DNA (about 60 base pairs). H1 binds both to the DNA of the core portion and also to the linked DNA, which serves to link adjacent nucleosomes. Each of the five histones has its own corresponding autoantibody. Anti-DNA autoimmune reactions are linked to anti-histone autoimmune responses. Anti-histone antibodies are often detected in patients with anti-DNA antibodies, but anti-histone antibodies are not necessarily associated with anti-DNA antibodies. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: No relevant information. Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. Normal value Normal human anti-histone antibodies are negative. Clinical significance In patients with systemic lupus erythematosus (SLE), the anti-histone antibody detection rate is 30% to 70%, but it is not related to the disease activity and clinical manifestations; in patients with uncompressed rheumatoid arthritis (RA) The positive rate was 15%, and in patients with Felty syndrome, it was 83%, the positive rate of RA-related vasculitis patients was 75%, and the positive rate of young patients with rheumatoid arthritis (JRA) was 60%. In non-rheumatic autoimmune diseases, approximately 76% of patients with primary biliary cirrhosis are positive for anti-histone antibodies, and most are anti-H1 antibodies. Many drugs can induce a syndrome similar to SLE, called drug-induced lupus, the condition is lighter than SLE, and the symptoms can disappear after stopping the drug. The detection rate of anti-histone in patients with drug-induced lupus is high (>95%). In patients with lupusamide-induced lupus, antibodies against H2A-H2B histone dimers are mainly present. In patients with lupus induced by indextrin, anti-H2 and anti-H4 antibodies are mainly present. In patients taking procainamide for more than 1 year, up to 20% of drug-induced lupus occurs; 5% to 10% of patients taking querxazine can induce lupus. In addition to these two drugs, dextromethorphan, D-penicillamine, isoniazid, quinidine, propylthiouracil, chlorpromazine, acetophenoxytoxin and methyldopa can also induce lupus. The diagnosis of drug-induced lupus is strongly supported when only anti-histone antibodies (and anti-ssDNA antibodies) are detected in the patient's serum without other anti-nuclear antibodies. The anti-H2A-H2B dimer IgG antibody is closely related to the clinical activity of the disease. Positive results may be diseases: systemic lupus erythematosus scleritis, systemic lupus erythematosus-induced myelopathy, systemic lupus erythematosus, nephrotic syndrome, drug-induced lupus, lupus erythematosus Inappropriate people: generally no special population. Taboo before inspection: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. Requirements for examination: When taking blood, you should relax your mind, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection. Cooperate with the doctor to do all the checks. Inspection process The serum of the patient was tested for anti-total histone and histone subunit antibodies by enzyme-linked immunosorbent assay (ELISA) and immunoblotting techniques. Not suitable for the crowd There are no special taboos. Adverse reactions and risks There are no related complications and hazards.