Antineutrophil antibody (ANGA)

"Anti-neutrophil antibody" (ANGA) is commonly referred to as antibodies in the serum of patients with neutropenia and antibodies against neutrophil membrane components, these autoantibodies and "anti-neutrophil cytoplasmic antibodies" (ANCA) different. Determination of ANGA can assist in the diagnosis of various clinically granulocytopenic diseases. Need to check the crowd is more common in multiple system autoimmune disorders often accompanied by autoimmune neutropenia. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Indirect immunofluorescence, ELISA: negative. Positive: Suffering from non-specific systemic necrotizing small vasculitis, primary small vascular inflammatory renal damage, leukopenia and agranulocytosis. Tips: After 8 pm on the day before the medical examination, you should start fasting for 12 hours. Normal value Indirect immunofluorescence, negative for ELISA. (Note the specific reference value depends on each laboratory.) Clinical significance Abnormal results Viral gold disease, non-Hodgkin's disease and chronic lymphocytic leukemia virus infection may be associated with immune neutropenia, especially EB virus and HIV infection, neutrophil antibodies appear In the non-symptomatic phase of HIV infection and in the symptomatic period, a large increase. Need to check the crowd is more common in multiple system autoimmune disorders often accompanied by autoimmune neutropenia. Positive results may be diseases: leukopenia and neutropenia precautions Abnormal people: generally no special population. Forbidden before examination: Please inform the doctor about the recent medication and special physiological changes before the test. 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. Requirements for examination: When taking blood, you should relax your mind, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection. Inspection process In the first step, an unknown unlabeled antibody (sample to be tested) is added to a known antigen sample, and incubated at 37 ° C for 30 min in a wet box to sufficiently bind the antigen antibody, followed by washing to remove unbound antibody. In the second step, a fluorescently labeled anti-globulin antibody or an anti-IgG, IgM antibody is added. If an antigen-antibody reaction occurs in the first step, the labeled [de]antiglobulin antibody will further bind to the antigen-binding antibody, thereby identifying an unknown antibody. Not suitable for the crowd There are no special taboos. Adverse reactions and risks There are no related complications and hazards.