anti-insulin receptor antibody

The anti-insulin receptor antibody (IRA) was found by Flier in 1975 when studying patients with insulin resistance syndrome with acanthosis nigricans. This antibody binds to the insulin receptor present on the cell membrane of the body and exhibits a decrease in affinity for insulin. When IRA is detected, it is commonly used to pass human lymphocytes IM-9 (rich receptors on the cytoplasmic membrane, cells are readily available) or human placenta to prepare insulin receptor antigens. Common methods are Radioreceptor Analysis (RRA) and immunoprecipitation tests. Basic Information Specialist classification: Digestive examination classification: endocrine examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Diabetic patients. Tips: Before the inspection taboo: should not eat foods with high sugar content, will directly affect the test results. Normal value Insulin receptor antibody (IRA) serum to be tested for inhibition of 125I-insulin binding to receptors Serum dilution <1:2 (radio-receptor assay/immunoprecipitation assay). Clinical significance Abnormal results: The presence of this antibody can result in a significant decrease in the affinity of the insulin receptor for insulin, allowing diabetics to require high doses (25,000 U) of insulin per day to control blood glucose. Patients with unstable diabetes have lower IRA levels, and those with hyperplastic retinopathy and kidney disease have higher IRA levels. Diabetes patients with insulin resistance syndrome, proliferative retinopathy and kidney disease need to be examined. Positive results may be diseases: considerations for diabetes Taboo before inspection: It is not advisable to eat foods with high sugar content, which will directly affect the test results. Requirements for examination: When the serum to be tested contains anti-insulin antibodies at the same time, the results of the anti-insulin receptor antibody (IRA) are interfered with and need to be corrected by other methods. Inspection process ELISA method: operation steps Coated with purified human, pig or bovine crystalline insulin, the test serum is diluted 1:10, plus the optimal amount of working concentration of HRP-labeled anti-human IgC antibody or labeled anti-human IgA F(ab'): bottoming The color was developed and the positive result was tested for the highest dilution of serum AIAb. Not suitable for the crowd Not suitable for the crowd: generally no special population. Adverse reactions and risks There are no related complications and hazards.