Anti-Parietal Cell Antibody (APCA)

Anti-gastric cell antibody is an antibody against granules in the cytoplasm of gastric parietal cells, mainly IgG type, and also IgA type antibody. Anti-parietal cell antibody (PCA) was first discovered in the serum of patients with pernicious anemia by Taylor et al. The target antigen is localized in the membrane of the parietal microvilli, which is a microsome part of the cytoplasm and a cytoplasmic membrane. lipoprotein. PCA is organ-specific and does not react with other organs other than the stomach, but has no species specificity. Therefore, rat or rabbit gastric parietal cells can be used as antigens. The Ig category of PCA is mainly IgG and IgA (also a small amount of IgM), IgG is the main serum, and IgA is more common in gastric juice. Basic Information Specialist classification: Digestive examination classification: blood examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Positive in malignant anemia (positive rate of 80%), chronic hypohemolytic anemia, simple atrophic gastritis and gastric cancer. In addition, hyperthyroidism, primary hypoparathyroidism, primary adrenal atrophy, etc. can also be positive, but the positive rate is not high (10% to 30%). Tips: Take a large number of drugs that affect antibodies against gastric parietal cells before the test. Normal value ≤1:10. Clinical significance Positive in malignant anemia (positive rate of 80%), chronic hypohemolytic anemia, simple atrophic gastritis and gastric cancer. In addition, hyperthyroidism, primary hypoparathyroidism, primary adrenal atrophy, etc. can also be positive, but the positive rate is not high (10% to 30%). Positive results may be diseases: chronic gastritis, gastric ulcer precautions Pre-examination contraindications: Take a large number of drugs that affect antibodies against gastric parietal cells. Requirements for examination: 2ml of venous blood, no anticoagulation, separation of serum for determination. This test has nothing to do with the positive gender. During the examination, the patient pays attention to the doctor and waits patiently. Inspection process The conventional detection method is an IIF method in which a mouse stomach frozen section is used as an antigen matrix. The fluorescent pattern is dyed in the fine granules of the cytoplasm of the parietal cells, and is often strongly stained at the apex. The mouse stomach is a suitable substrate because a false positive reaction occurs in the stomach of the rat. An ELISA established by antigen purification by affinity chromatography is also a preferred method. Not suitable for the crowd Inappropriate crowd: no special population. Adverse reactions and risks No special complications.