Anti-zona pellucida antibody (AZP)

The anti-clear zone antibody (AZP) is an autoantibody produced by a woman in her own zona pellucida tissue and has anti-fertility ability. Clinically, ELISA and immunofluorescence methods are commonly used. The ELISA method is described below. The ELISA method is a highly sensitive test technique that combines the specific reaction of antigens and antibodies with the efficient catalytic action of enzymes on substrates based on immunological reactions. The reaction of the antigen and the antibody is carried out in a well of a solid phase carrier, a polystyrene microtiter plate, and after each reagent is added, the excess free reactant can be removed by washing to ensure the specificity of the test result. stability. Basic Information Specialist classification: gynecological examination classification: immune examination Applicable gender: whether women are fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Lead to female infertility. Tips: Menstrual women are not suitable for this check. Normal value ELISA and immunofluorescence were negative. Clinical significance Positive may be one of the reasons for female infertility. AZP can restore fertility when it is turned negative. Positive results may be diseases: infertility precautions When inspecting: The inspection process needs to be carried out in conjunction with a doctor. Inspection process Blood is taken to see if the immunoglobulin in the blood is specific. This antibody is increased. After examination, if there is specificity, it will increase to a certain proportion. If the pig egg zona pellucida (PZP) is used as an antigen to detect AZP in human serum, the serum to be tested must be absorbed by porcine red blood cells; if human egg ZP is used as the antigen, the serum to be tested must be absorbed by AB type human red blood cells, otherwise false Positive reaction (due to the presence of heterophilic lectin in normal human serum). Not suitable for the crowd Menstrual women are not suitable for this examination. Adverse reactions and risks No special complications.