Anti-Lymphocyte Antibody (ALA)

The anti-lymphocyte antibody detection step uses a plastic immunoreaction plate, and 5 μl of liquid paraffin is added to each well in turn, 1 μl of the patient's serum, 1 μl of normal human lymphocyte suspension, placed in a 20 ° C incubator for 30 min, and then added with fresh rabbit complement 5 μl in a thermostat for 60 min. Then, add iyihong-Y2.5μl for 3~5min, and then fix it with 37% formaldehyde 3μl for more than 5h. Each well was tested in parallel for 3 times, and finally the results were observed under a phase contrast microscope. Judging criteria: 1. Negative Under the phase contrast microscope, the lymphocytes in the reaction well are reddish, the cell membrane is smooth and the refractive index is very strong. 2. The lymphocytes in the positive reaction well are 60% to 100%. Swelling and degeneration, large and flat, dull, and the membrane is rough and loses its refractive power. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: Normally negative. Positive: Positive systemic lupus erythematosus (SLE). Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. Normal value negative. Clinical significance Positive systemic lupus erythematosus (SLE). Precautions First, the precautions before blood draw: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Second, after blood draw should pay attention to: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Third, attention: pregnancy, blood transfusion, organ transplantation and other conditions are also induced. 4. Please inform the doctor about the recent medication and special physiological changes before the test. Inspection process Same as the micro-lymphocyte method. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.