Antistreptolysin "O" test

Referred to as anti-"0" or ASO test. Streptococcal hemolysin is one of the metabolites of hemolytic streptococcus. Has hemolysis and antigenicity. 2-3 weeks after the human is infected with hemolytic streptococcus, an antibody against streptococcal o is produced in the body. This experiment is based on the principle of antigen-antibody neutralization test, and the presence or absence of neutralizing antibody production in serum is tested by reducing hemolysin o having hemolysis ability. Where the titer of the sub-antibody in the serum of the patient is significantly increased, more than 400 units, the patient may be considered to have recently been infected with hemolytic streptococcus, and is used to assist in the diagnosis of patients with rheumatic fever and glomerulonephritis. Basic Information Specialist classification: cardiovascular examination classification: immune examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Prompt hemolytic streptococcal infection. Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. After 8 pm on the day before the medical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. Normal value ASO<400U. Clinical significance 1 anti-"O" value of more than 400 units, suggesting that there has been hemolytic streptococcal infection. Therefore, diseases caused by this bacterial infection (such as scarlet fever, erysipelas, acute nephritis, etc.) will increase the anti-"O" value. Because of the non-specificity of anti-"0" and erythrocyte sedimentation rate, even if the patient's anti-zero and erythrocyte sedimentation rate increase, the diagnosis of active rheumatism should be considered in combination with clinical manifestations. 2 Some diseases that have no obvious relationship with hemolytic streptococcus can also increase the anti-"O" value. Such as a small number of hepatitis, nephrotic syndrome, tuberculosis, connective tissue disease, subacute infective endocarditis and some patients with allergic purpura, the differential diagnosis should be combined with clinical data. 3 Hypercholesterolemia, macroglobulinemia, multiple myeloma patients, ASO can also increase. Precautions First, the precautions before blood draw: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the physical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection; If you have a history of fainting blood, please explain in advance and make special arrangements. Second, after blood draw should pay attention to: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of dizziness, such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of sugar water, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Inspection process Serum samples were diluted to two dilutions of 1:100 and 1:500 with ASO buffer. Dilute with a reducing agent solution to about 1/3 of the final volume, leave it for 15 minutes, fully reduce the hemolysin, then accurately dilute to the specified volume and use it within 45 minutes. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.