Anti-Scl-70 antibody

Douvas is equivalent to the 1979 report that there is an anti-nuclear antibody in the serum of patients with scleroderma whose target antigen is a non-histone protein in the nucleus due to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular weight of the medium shift is 70kD, so it is called Scl-70. In 1986, Shero et al. confirmed that the essence of Scl-70 is DNA topoisomerase-1, which has a natural molecular weight of 100 kD and a 70 kD antigen as its degradation fragment. DNA topoisomerase-1, located in the nucleoplasm, has a high concentration in the nucleolus. It is involved in the process of DNA supercoil release and plays a role in the replication and transcription of DNA duplexes. This enzyme cleaves the DNA strand and attaches to the free end produced, so that the entire DNA molecule is not required to unwind, but only in the region to be replicated. Once a region has been replicated or transcribed, the DNA duplex is recombined and the topology enzyme is released. It is a key protein with important biological functions in cells. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Normal humans are negative for anti-Scl-70 antibodies. Positive: Abnormal results were positive, mainly seen in sclerosis (PSS), also seen in CREST syndrome. Tips: When you take blood, you should relax your mind, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection. Normal value Normal humans are negative for anti-Scl-70 antibodies. Clinical significance Abnormal results were positive, mainly seen in sclerosis (PSS), also seen in CREST syndrome. Patients who need to be examined for unexplained organ fibrosis. Precautions Before the test: Do not eat too greasy, high-protein food the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. When checking: When you take blood, you should relax your mind, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection. Inspection process After taking the blood, hand it to the doctor for examination. Actively cooperate with doctors. Not suitable for the crowd Not suitable for people: people with very low platelets or hemophilia patients. Adverse reactions and risks There are no related complications and hazards.