Anti-Ku antibody

Anti-Ku antibodies were reported by Mimori in 1981. They found that a patient with systemic sclerosis/polymyositis overlap syndrome (Ku) could precipitate with a DNA-binding protein. This nuclear protein is called Ku antigen and is sensitive to trypsin but not by DNase and RNase; heating (37 ° C for 30 min or 56 ° C for 15 min) and pH change (pH ≥ 10 or < 6) can cause its antigenic loss. The protein portion of the Ku antigen consists of a heterodimer composed of p70 and p80 (molecular weight 70kD and 80kD or 66kD and 86kD, respectively), which bind non-covalently to dsDNA ends, gaps and gaps (gapsandnicks) and participate in DNA fragmentation. After the repair. Ku protein is a DNA-binding portion of DNA-dependent protein kinase that catalyzes the phosphorylation of RNA polymerase II and other transcription factors. Ku antigen is widely present in human, calf, and rabbit tissues. The molecular weight of the crude Ku antigen was 300 kD by gel filtration, and the purified Ku antigen was 173±21 kD by polyacrylamide gel electrophoresis. The molecular weight estimated by electron microscopy was about 160 kD. Human anti-Ku antibodies do not react with guinea pig, rat, mouse cells. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: Anti-Ku antibodies were negative in normal human serum. Positive: If the serum is positive for anti-Ku antibodies, it can lead to systemic sclerosis, polymyositis, other connective tissue diseases such as rheumatoid arthritis (RA), mixed connective tissue disease (MCTD), and Sjogren's syndrome. Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. Normal value Anti-Ku antibodies were negative in normal human serum. Clinical significance Abnormal results If the serum is anti-Ku antibody positive, it can lead to systemic sclerosis, polymyositis, other connective tissue diseases such as rheumatoid arthritis (RA), mixed connective tissue disease (MCTD), and Sjogren's syndrome. Patients who need to be examined for muscle weakness, muscle tenderness, joint synovial lesions, etc., are suspected of multiple muscle inflammation, connective tissue disease and other patients. Precautions Before the test: Do not eat too greasy, high-protein food the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. When checking: When you take blood, you should relax your mind, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection. Inspection process After taking the blood, hand it to the doctor for examination. Actively cooperate with doctors. Not suitable for the crowd Not suitable for people: people with very low platelets or hemophilia patients. Adverse reactions and risks There are no related complications and hazards.