macrophage migration inhibition assay

The macrophage migration inhibition assay (MIT) is a method for determining the body-specific cellular immune response in vitro. The principle of MIT is that after the sensitized lymphocytes in the human body are exposed to the corresponding antigens, a lymphokine can be released to inhibit the normal movement of macrophages, and the function of the T lymphocytes can be understood by indicating the movement of the system. A shift index of less than 0.8 indicates that the lymphocytes have been sensitized to the added antigen, so there is a release of MIF under the action of the same antigen. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Tips: Before the test: Do not eat too greasy, high-protein foods, avoid heavy drinking. Normal value A shift index of less than 0.8 indicates that the lymphocytes have been sensitized to the added antigen, so there is a release of MIF under the action of the same antigen. Clinical significance (1) Reduce the body's specific effects against the original. (2) Elevated antigen has a stimulating effect on macrophages or another specific immune response exists in the body. Precautions Before the test: Do not eat too greasy, high-protein foods, avoid heavy drinking. When checking: When you take blood, you should relax your mind, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection. Inspection process Immediately after the blood is drawn, the disinfected dry cotton block (disinfecting the wet cotton block is easy to stimulate the puncture point to cause pain, and is not easy to stop bleeding). The puncture site is pressed, and the local compression is required in the range of the pinhole and the upward two-inch needle. - 5 minutes to stop bleeding. Do not be embarrassed, so as not to cause subcutaneous hematoma. Thereafter, the blood-sucking arm should be kept clean and hygienic within 24 hours, without showering or sauna. After taking the blood, take a rest for 15 minutes. It is best not to move within 15 minutes. Sit down or lie down. If symptoms such as dizziness, vertigo, fatigue, etc. should be immediately lying down, drink a small amount of sugar, wait until the symptoms are relieved. Conduct a medical examination. Not suitable for the crowd Not suitable for people: When the platelets are extremely reduced, there are also coagulopathy, such as hemophilia. Adverse reactions and risks There are no related complications and hazards.