Glutamate decarboxylase antibody (GADA)

Anti-glutamate decarboxylase antibody (GADA) is a hallmark antibody for type 1 diabetes and is used for the diagnosis and differential diagnosis of progressive or occult type 1 diabetes. In addition, studies have shown that GABA plays an important role in brain development and function, and may be associated with certain neurological diseases, GAD-associated autoimmune sclerosis syndrome. GADA has two kinds of 65 kDa GADA65, 67 kDa GADA65, and is commonly used in clinical ELISA. ELISA is a highly sensitive test technique that combines the specific reaction of antigens and antibodies with the efficient catalysis of enzymes on substrates based on immunological reactions. Basic Information Specialist classification: neurological examination classification: immune examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: Normally negative. Positive: Abnormal results, the positive rate of GADA in the early stage of type 1 diabetes was 38-76%, and the positive rate in class I relatives was 78-81%, almost all of them were GADA65. The distribution of GADA67 is very small, and the positive rate of type 2 diabetes is only 0~4%. Tips: After the blood draw, symptoms such as dizziness, vertigo, fatigue, etc. should be immediately supine, drink a small amount of sugar water, and then undergo a physical examination after the symptoms are relieved. Normal value Negative ELISA. Clinical significance Positive: The positive rate of GADA in the early stage of type 1 diabetes is 38-76%, and the positive rate in class I relatives is 78-81%, almost all of which is GADA65. The distribution of GADA67 is very small, and the positive rate of type 2 diabetes is only 0~4%. Note that the detection rate of ICA and GADA in patients with type 1 diabetes is positively correlated. The agreement rate of the two antibodies is 90% in the early stage of the disease and 54% in the disease process. Precautions Before inspection: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. After inspection: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. Inspection process Inspection method: draw blood. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.