Bacteriological examination of eye specimens

Bacteriological examination of eye specimens is to identify bacteria in the eye, such as Chlamydia trachomatis antigen, corneal lesions and other methods. Corneal lesion smear examination for acanthamoeba keratitis, lesion tissue wet film examination can observe the acanthamoe cyst, is an important means of diagnosis and discovery of Acanthamoeba keratitis. Specific antibodies can be detected in the serum of patients infected with Chlamydia trachomatis. Basic Information Specialist Category: Ophthalmology Classification: Biochemical examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: The type and proportion of the eye flora are normal, and the human body is in a state of dynamic balance and health. Chlamydia trachomatis is negative. Positive: lgM appeared earlier, lasting about 1 month, and its positive suggestive of recent infection with Chlamydia trachomatis can be used as an early diagnosis indicator; lgG appears later and lasts longer, and can be used for retrospective diagnosis and epidemiological investigation. Tips: Pregnant women can be treated with erythromycin if they are infected with Chlamydia trachomatis. Normal value The type and proportion of the eye flora are normal, and the human body is in a state of dynamic balance and health. Chlamydia trachomatis is negative. Clinical significance Corneal lesion smear examination for acanthamoeba keratitis, lesion tissue wet film examination can observe the acanthamoe cyst, is an important means of diagnosis and discovery of Acanthamoeba keratitis. Specific antibodies can be detected in the serum of patients infected with Chlamydia trachomatis. lgM appeared earlier, lasting about 1 month, and its positive suggestive of recent infection with Chlamydia trachomatis can be used as an early diagnosis indicator; lgG appears later and lasts longer, and can be used for retrospective diagnosis and epidemiological investigation. Abnormal results of purulent keratitis, corneal perforation and endophthalmitis, herpes simplex keratitis, fungal keratitis; Chlamydia trachomatis antigen found in trachoma, adult inclusion body conjunctivitis, male urethritis, female cervicitis and salpingitis, sexually transmitted diseases Lymphocytic granuloma and the like. The people who need to be examined have the above symptoms such as suppurative keratitis, corneal perforation and endophthalmitis, and herpes simplex keratitis. Precautions (1) Eliminating sexual disorder is the most important preventive measure for both Chlamydia trachomatis infection and other sexually transmitted diseases. The style of life is rigorous, not involved in fornication, and not involved in extramarital sex, in order to prevent problems before they occur. (2) Pay attention to personal hygiene: use personal toiletries and towels alone; do not wear other people's underwear and swimwear; do not wash the pond during the outing; try to use the sitting toilet and wash your hands before going to the toilet. These practices can reduce the chance of exposure to infection. (3) Spouse should be prohibited from sexual life during infection with Chlamydia trachomatis. Modern medicine has many drugs to treat Chlamydia trachomatis infection, and it is convenient to use. Patients only need to adhere to treatment and will soon return to health. Treatment should be carried out simultaneously between husband and wife. Women may have no obvious symptoms when they are sick. Do not refuse treatment because they have no symptoms and become a concealed person. (4) Pregnant women can be treated with erythromycin if they are infected with Chlamydia trachomatis. After the treatment, review and confirm that the baby can be delivered from the vagina. Inspection process 1. For fungal keratitis, take a small amount of diseased tissue from the ulcer and make a 10% KOH wet film for direct microscopic examination. If the hyphae are found, the fungal keratitis can be quickly diagnosed. The positive rate of microscopic examination in many large specialized hospitals is 50%. ～90%. 2. Before use, please use ACONChlamydia test kit and return the lysate to room temperature (15-30 °C). 1. Use the kit as soon as possible after removing it from the sealed aluminum foil strip to avoid moisture. 2. Place the kit on a clean water platform. 3. Obtain Chlamydia trachomatis antigen according to the type of specimen. Cervical and urethral specimens: A 5 ml of colorless lysate (approximately 300 μl) was dropped vertically into the lysing tube, immediately placed in the patient's cotton swab, the lysing tube was squeezed, and the cotton swab was rotated 10 times. The cotton swab was then allowed to stand in the lysis tube for 2 minutes. A lysate B7 drop (about 300 μl) was dropped vertically into the lysis tube, and the mixture produced a precipitate. Squeeze the cracking tube and turn the cotton swab 10 times until the color of the solution turns pale blue. If the cotton swab is bloody, the solution will be yellow. Allow to stand for 1 minute. Squeeze the cotton swab and discard the cotton swab. Plus the dripper. Urine sample: A5 drops (about 300 μl) of lysate was dropped vertically into the centrifuge tube, and vortexed and mixed (if necessary, vortexed with a vortex mixer) until the sample solution was uniform. Allow to stand for 2 minutes. A lysate B7 drop (about 300 μl) was dropped vertically in a centrifuge tube, shaken and mixed, and allowed to stand for one minute. 4. Drip 3 drops of the lysed solution (about 100 μl) into the well of the kit. Read the results at 5.5-10 minutes.