Brucella agglutination test

The anti-Breucoid antibody in the serum to be tested meets the latex reagent, and an antigen-antibody reaction occurs, and a macroscopic agglutination reaction occurs. Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. Basic Information Specialist classification: Infectious disease examination and classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Tips: If you have a history of fainting blood, please explain in advance, and make special arrangements. Normal value The agglutination titer is less than 1:40. Clinical significance This project is used for the diagnosis of brucellosis. The disease is one of zoonotic diseases, and humans are infected by direct contact with the carrier animals. The non-endemic area agglutination titer is generally greater than 1:80 for diagnostic significance; the epidemic area and pastoral area agglutination titer above 1:160 is diagnostic. The antibody titer of acute Brucella infection increased rapidly in the second week of the disease, peaked at 3 to 6 weeks (1:640 or higher), and the high titer could be maintained for about 1 year, then decreased significantly, if the titer rose again. , suggesting reinfection or recurrence. Precautions First, the precautions before blood draw: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the physical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Second, after blood draw should pay attention to: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of dizziness, such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of sugar water, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Inspection process The serum to be tested (diluted 1:25, 1:50, 1:100, 1:200, 1:400, 1:800 dilution with physiological saline) was 0.5 ml, and 0.5 ml of the diagnostic bacterial solution was added thereto, and the mixture was mixed. The results were observed in a 37 ° C water bath for 16-20 h. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.