Paragonimus metacercariae membrane reaction

Paragonimiasis (also known as paragonimiasis) is caused by Paragonimus of the trematode. There are many types of paragonimiasis. In China, the main pathogens are Paragonimus sinensis and Schistosoma japonicum. The human beings are suitable hosts and non-appropriate hosts of Paragonimus sinensis and Paragonimus sinensis. After the cystic mites infect humans, Paragonimus sinensis develops into adults through the larvae; while Paragonimus sinensis is developed and retained. In the stage of larvae, the former mainly causes pulmonary paragonimiasis, and the latter mainly causes subcutaneous paragonimiasis. Paragonimiasis often involves multiple organs such as the lungs, brain, liver, and skin. The symptoms are more complicated. Paragonimiasis has the common and specific antigenic components of the species and the common antigen components in the period, and the common antigen components are used to facilitate the diagnosis of paragonimiasis. For example, two paragonimus antigens can be substituted for each other to detect different species. Fucoccosis; specific antigenic components can be used to further identify the species or stage. When a specific antibody in serum is detected by the crude antigen of Paragonimus, cross-reaction occurs with schistosomiasis and liver fluke, and the cross-reaction can be reduced or eliminated by using different purified antigens. The results of animal experiments showed that the appropriate host first appeared anti-cysticercosis antibody in the serum 10 days after infection, and then the titer gradually increased to 30-80d, and then gradually decreased to low level after 90d; The antibody appeared at 40 days after infection, reached a peak at 80 days, and decreased at 90-100 days, and maintained a low level for a long time with a slight increase. In non-appropriate hosts, anti-capsule antibodies appeared on the 15th day after infection, and the titer continued to rise after reaching the peak at 80d, and remained at the high titer antibody level afterwards; however, the antibody response to adults was weak and appeared. late. After effective treatment, patients with paragonimiasis showed an increase in antibody levels between the first and second months, and between the third and sixth months, antibodies decreased. Basic Information Specialist classification: Infectious disease examination and classification: immunological examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: After the negative reaction, there was no reaction around the cercaria body. Positive: Positive indicates schistosomiasis infection. Tips: According to the nutritional status of the whole body, milk, eggs, fruits, soy milk, etc. are given in the meal. Normal value After the negative reaction, there was no reaction around the cercaria body. Clinical significance The positive rate of detection is 76.47%～88%, which has obvious cross-reaction with schistosomiasis patients (7%) and liver fluke patients, and can be used for early diagnosis. Precautions The posterior tibial membrane reaction method is simple, sensitive, and specific, and can be used for early diagnosis of paragonimiasis. The disadvantage is that the antigen is not easy to obtain and preserve, and it is still difficult to promote and apply. Inspection process The paragoniminis cyst was isolated from the infected second intermediate host, placed in a 20 g/L cholate solution, and incubated at 40 ° C for 1 h. Take 5 off-capsules and place them in a concave glass slide. Add 3 drops of serum to the test subject, seal with a cover glass, and incubate in a wet box at 37 °C for 24 hours. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.