Mycobacterium tuberculosis antigen and antibody test

The antigen and antibody test of Mycobacterium tuberculosis is carried out by immunological methods. The pathogenic effect of Mycobacterium tuberculosis may be related to the inflammatory reaction caused by the invigorating proliferation of bacteria in tissue cells and the induction of delayed type allergic damage in the body. Mycobacterium tuberculosis can enter the body through the respiratory tract, digestive tract and damaged skin and mucous membranes, invading various tissues and organs, causing the corresponding organs, causing tuberculosis of the corresponding organs, among which tuberculosis is the most common. Basic Information Specialist classification: Infectious disease examination and classification: immunological examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: The results of antigen and antibody tests were all negative. Positive: 1. Cause lymphangitis and lymphadenopathy, X-ray chest radiograph shows dumbbell-shaped shadow, this is the first infection with Mycobacterium tuberculosis, more common in children. 2. Chronic granulomatous inflammation, formation of tuberculous nodules, fibrosis or caseous necrosis. The lesion often occurs at the tip of the lung. More common in adults. Tips: Pay attention to normal eating habits and pay attention to personal hygiene. Normal value The results of antigen and antibody tests were all negative. Clinical significance Abnormal result 1. Cause lymphangitis and lymphadenopathy, X-ray chest radiograph shows dumbbell-shaped shadow, this is the first infection with Mycobacterium tuberculosis, more common in children. 2. Chronic granulomatous inflammation, formation of tuberculous nodules, fibrosis or caseous necrosis. The lesion often occurs at the tip of the lung. More common in adults. People who need to be examined: Patients with suspected systemic miliary tuberculosis, tuberculous meningitis, chronic granulomatous inflammation, tuberculous nodules, and fibrosis or caseous necrosis. Or (1) clinical manifestations of meningitis; (2) CSF biochemistry consistent with changes in the brain; (3) some cases with extracranial tuberculosis; (4) effective anti-tuberculosis treatment. Positive results may be diseases: pediatric renal tuberculosis, pediatric primary tuberculosis, pediatric herpes keratoconjunctivitis, sputum, pediatric mesenteric lymph node tuberculosis, tubal tuberculosis, chest wall tuberculosis, fistula, nontuberculous mycobacterial disease, penile tuberculosis Precautions The test results show that if a single antigen or antibody is detected, a small number of missed diagnosis will result. Parallel detection of antigens and antibodies can compensate for false negatives in a single test and reduce missed diagnosis. Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Requirements for inspection: Actively cooperate with the doctor. Inspection process Mycobacterium tuberculosis antibody test kit: Tuberculosis In different stages of disease development, tuberculosis antigens recognized by T lymphocytes are not the same. The most commonly used diagnostic tuberculosis-specific antibody is the 38kD antigen. The anti-38kD antibody is the highest antibody positive rate among smear-positive tuberculosis patients. It is undoubtedly the best antigen for single-diagnosis tuberculosis so far, and it is a tuberculosis infection. An important antigen for later detection. The 16kD antigen is suitable for patients with early tuberculosis infection and can detect active tuberculosis as soon as possible. This product uses the indirect method to detect 38kDa protein and 16kDa protein antibody in human serum samples for clinical diagnosis of active tuberculosis. [Principle] The solid phase M. tuberculosis Tb-38-16 antigen on the spot reaction plate forms a complex with the M. tuberculosis antibody in the serum, and the colloidal gold-labeled anti-human IgG antibody is combined with the complex to form a macroscopic view. A red round spot is a positive result, otherwise it is a negative result. 【Testing method】 1. Restore the serum sample and kit (especially reagents A and B in the kit) to room temperature (25-37 ° C); 2. Take out the reaction plate, drop two drops of reagent A into the well of the reaction plate, and let stand, until the reagent A is completely inhaled; 3. Using a night machine to absorb 100 microliters of the serum sample to be tested in the well of the reaction plate, and let stand, until the serum is completely inhaled; 4. Remove the blue ear cap on the reaction plate, add three drops of reagent B, and let stand, until the reagent B is completely inhaled; 5, then add two drops of reagent A, let stand, until the reagent A is completely inhaled, observe the phenomenon in the well of the reaction plate within 5 minutes; [Explanation of test results] If the test result is positive, the detection of Mycobacterium tuberculosis antibody is positive, and the negative one is the detection of Mycobacterium tuberculosis antibody. METHODS: Detection of tuberculosis antigen: BCG was added by ultrasonic pulverization (BCG) plus PPD, and rabbits were immunized 6 times with Freund's complete adjuvant. After 2 months, antiserum was obtained. The protein content was measured after salting out (NH4)2SO4 twice. Horseradish peroxidase was labeled with sodium periodate and titrated at a working concentration of 1:80. The antibody coating concentration was 50 μg/ml. The tuberculosis antigen in CSF was measured by double-antibody sandwich method. Detection of anti-PPD antibody IgG: The coated PPD concentration was 10 μg/ml, and the anti-PPD antibody IgG was measured indirectly by ELISA. The experimental results were measured by an enzyme-labeled spectrophotometer at 492 nm absorbance [A, formerly called optical density (OD)]. After all the test data were statistically processed, A≥0.20 was used as a positive result. Tuberculosis antigen and anti-pure protein derivative (PPD) antibody IgG in cerebrospinal fluid. Not suitable for the crowd Inappropriate crowd: No. Adverse reactions and risks Nothing.

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