α2-plasmin inhibitor activity

The α2 plasmin inhibitor is mainly synthesized by the liver, a single-chain glycoprotein, which is a serine protease with specific inhibitory activity in vivo. It inhibits the action of plasmin in a limited time and inhibits the binding of plasminogen to fibrin to prevent fiber. The protein is hydrolyzed by antiplasmin. Basic Information Specialist classification: Oncology examination classification: immune examination Applicable gender: whether men and women apply fasting: not fasting Tips: At a certain temperature, the accuracy of the colorimetric volume should be guaranteed. Before the analysis. It is best to pre-warm the mixing reagent and keep the plasmin at 15 to 25 °C. During the reaction, the substrate releases p-nitroaniline, which is a toxic substance and should be protected from skin contact or inhalation. Normal value 0.8 to 1.2 inhibition units/ml. Clinical significance In the venous and arterial thrombosis, malignant tumors and postpartum α2-antiplasmin increased; α2-antiplasmin was reduced in liver disease, DIC, postoperative and congenital deficiencies. Precautions (1) At a certain temperature, the accuracy of the colorimetric volume should be ensured. Before the analysis. It is best to pre-warm the mixing reagent and keep the plasmin at 15 to 25 °C. (2) The p-nitroaniline released by the substrate during the reaction, which is a toxic substance, should avoid skin contact or inhalation. If the solution comes into contact with the skin mucosa, rinse immediately with plenty of water. Inspection process After mixing, incubate for 30 min and colorimetric at 405 nm at room temperature. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.