T cell transformation assay

Also known as the T cell rejuvenation test. When cultured in vitro, T cells can be transformed into large-sized mother cells after being stimulated by mitogens such as phytohemagglutinin (PHA). After 8 pm on the day before the medical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: not fasting Tips: If you have a history of fainting blood, please explain in advance, and make special arrangements. Normal value 60.l ± 7.6%. Clinical significance E-ring value reduction: 1 found in immunodeficiency diseases (such as Digeorge syndrome, Wiskott-Aldrich syndrome, Nezelof syndrome) and primary cell and humoral immunity simultaneously lacking disease. When these diseases are treated with thymosin or the like, EaRFC can be significantly increased. 2 Some malignant tumors, measles, measles encephalitis, mumps, herpes zoster, systemic lupus erythematosus, dermatomyositis, lepromatous leprosy, and the use of immunosuppressants also see a decrease in E-ring values. Some people think that some malignant tumors EaRFC can be earlier than the clinical manifestations of the disease, and EtRFT can still be normal. E garland value increases: Found in hyperthyroidism, thyroiditis (which can return to normal when the two conditions are relieved), myasthenia gravis, chronic active or persistent hepatitis, chronic lymphocytic leukemia, and transplant rejection. Precautions First, the precautions before blood draw: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the physical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Second, after blood draw should pay attention to: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of dizziness, such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of sugar water, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Inspection process MTT participates in the reaction as a substrate, and the blue formazan particles are deposited in or around the cells. After being dissolved in hydrochloric acid-isopropanol, the solution is blue. The OD of the cell culture can be determined by an enzyme labeling instrument. Value, measurement wavelength 570 nm. The degree of cell proliferation in the reaction system was calculated based on the magnitude of the OD value. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.