T cell rosette formation assay

The surface of T cells has a receptor for sheep red blood cells (SRBC), which forms a wreath-like cell with SRBC, called red blood cell rosette formation or E rosette formation. Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: not fasting Tips: If there is local congestion, use a warm towel after 24 hours to promote absorption. Normal value EtRFC 64.4 ± 6.7%; EaRFC 23.6 ± 3.5%; EsRFC 3.3 ± 2.6%. Clinical significance E-ring value reduction: 1 found in immunodeficiency diseases (such as Digeorge syndrome, Wiskott-Aldrich syndrome, Nezelof syndrome) and primary cell and humoral immunity simultaneously lacking disease. When these diseases are treated with thymosin or the like, EaRFC can be significantly increased. 2 Some malignant tumors, measles, measles encephalitis, mumps, herpes zoster, systemic lupus erythematosus, dermatomyositis, lepromatous leprosy, and the use of immunosuppressants also see a decrease in E-ring values. Some people think that some malignant tumors EaRFC can be earlier than the clinical manifestations of the disease, and EtRFT can still be normal. E garland value increases: Found in hyperthyroidism, thyroiditis (which can return to normal when the two conditions are relieved), myasthenia gravis, chronic active or persistent hepatitis, chronic lymphocytic leukemia, and transplant rejection. Precautions First, the precautions before blood draw: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the physical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. If you have a history of fainting blood, please explain in advance, and make special arrangements. Second, after blood draw should pay attention to: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of dizziness, such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of sugar water, and then undergo a physical examination after the symptoms are relieved. Inspection process Take 2 ~ 3ml heparin anticoagulated venous blood (plus heparin 200U / ml anticoagulation), diluted 1 times with Hanks solution, and mix. Take 2 ~ 3ml lymphocyte separation solution into a 15mm × 150mm test tube, draw the diluted blood with a capillary dropper, add 1cm from the surface of the separation liquid, and gradually add the diluted blood to the stratified liquid (to avoid Rush into the stratified liquid). The volume ratio of diluted blood to stratified fluid was 2:1. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.