HBsAg immune complex

Immune complexes are antigen-antibody reaction products, which can be divided into two major categories, and immune complexes formed by specific immune complexes; non-specific immune complexes (such as in glomerulonephritis, systemic lupus erythematosus, etc.) . The determination of hepatitis B surface antigen immune complex has a certain significance for understanding chronic diseases of hepatitis B. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: not fasting Tips: After 8 pm on the day before the medical examination, you should start fasting for 12 hours, so as not to affect the test results. Normal value HBsAg immune complex is negative. (Enzyme-linked immunosorbent assay) HBsAg immune complex <116. (Measurement of polyethylene glycol precipitated gastric protein dissociation method) Clinical significance Positive: Prompt the body's response to hepatitis B virus. However, in some cases, immunological hepatocyte pathological damage may occur, and HBsAg immune complexes are deposited in a large number of tissues such as blood vessels and kidneys, which may cause an inflammatory reaction and an extrahepatic manifestation of hepatitis B. Precautions First, the precautions before blood draw: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Second, after blood draw should pay attention to: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. 3. Please inform the doctor about the recent medication and special physiological changes before the test. Inspection process Take the coated solid phase carrier reaction strips and place them on the frame and number them. 50 μl of serum to be tested was added to each well, and each plate was positive, negative and blank. Then, an enzyme-labeled anti-HBs1 drop was added to each well, and the micro-oscillator was shaken for 1 to 2 min, and placed in a 37 ° C water bath for 30 min. Remove the reaction plate, remove the liquid in the well, wash it with the washing liquid 5 times, each time to stand for 1 min, add the substrate solution (50 μl of liquid A and liquid B) after air drying, and develop color at 37 ° C for 10 min in the dark, with 2 mol. The /LH2SO41 drop terminates the reaction. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.