B factor

Factor B is a C3 activator precursor and is an important factor in the complement pathway activation pathway, which is mainly synthesized by the liver and macrophages. It is an important component involved in the activation of complement bypass, participates in the body's defense, and plays an important role in tissue and cell damage and inflammation. Basic Information Specialist classification: Oncology examination classification: immune examination Applicable gender: whether men and women apply fasting: not fasting Tips: Complement instability in serum is easy to destroy, can only be placed for 1 day at 4 °C, 5 days at 0 °C, and 1 month at -20 °C. Normal value 0.10 to 0.40 g/L. Clinical significance Increased in malignant tumors. Reduced in autoimmune hemolytic anemia, cirrhosis, chronic active hepatitis, acute glomerulonephritis. Precautions (1) Complement instability in serum is easily destroyed. It can only be placed for 1 day at 4 °C, 5 days at 0 °C, and 1 month at -20 °C. (2) The excessively high concentration of Mg2+ in the diluent will affect the activity of AP-CH50. (3) pH of the diluent: The optimum pH is 7.2 to 7.5, otherwise the results will be affected. (4) RE of different individuals have an effect on the measurement results. Inspection process (1) The same as the C3 content was determined, and an anti-B factor serum agar gel plate was prepared and punched. (2) Loading, the serum to be tested was diluted 1:5, and 10 μl was added to each well. (3) The wet chamber at 37 ° C was spread for 24 h. (4) Prepare a standard curve using the B factor standard. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.