6-ketoprostaglandin F1α

Prostaglandins are a group of unsaturated fatty acids composed of 20 carbon atoms. They were first discovered in semen, hence the name prostaglandin (PG). The PG consists of a five-carbon ring structure and two side chains. Its structure is divided into A, B, C, D, E, F, G, H, I and other types. The Arabic numerals in the lower right corner of the letter indicate the number of double bonds contained in the side chain of the PG molecule, such as PGE1 and PGE2. Substituents on the PG five-carbon ring are marked with α below the ring plane, such as PGF1α, and β above the ring plane, such as PGF2β. Different types of PG have different biological effects. At present, PGE1, PGE2, PGF2α, PGA2, PGI2, TXA2 and TXB2 are the most studied, among which the most widely studied are Pseudocyclin (PGI2) and Thromboxane (TXA2). Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Please relax when checking. Normal value Plasma (138 ± 77.9) ng / L. Clinical significance Changes in 6-keto-PGF1α levels are found in: 1 cardiovascular system: plasma 6-keto-PGF1α decreased in patients with atherosclerosis, TXB2 increased, diabetes, hyperlipidemia have similar changes. Elevated TXA2/PGI2 ratios tend to cause platelet aggregation, thrombosis, and promote arteriosclerosis and coronary heart disease. The level of 6-keto-PGF1α in the plasma of shock animals caused by bleeding, injury and endotoxin was increased. 2 Chronic renal failure: TXB2 and 6-keto-PGF1α decreased in the urine of patients. Intrarenal PG is important for regulating renal blood flow, and there are significant changes in urine in patients with renal vascular hypertension, nephrotic syndrome and Batter sign. 3PG is closely related to the reproductive system, especially the process of ovulation, corpus luteum, corpus callosum, uterine activity, and the operation of eggs and sperm. The increased PGI concentration in pregnant women and the reduced sensitivity to the angiotensin II pressor effect may be associated with increased PGI synthesis in the placenta. 4 inflammatory response: such as contact dermatitis patients in the skin eluate PGE and PGF content is 10 times higher than non-inflammatory skin. Inflammatory exudates also contain PGI2 and TXB2. Injection of exogenous PGE and PGI2 and other strong inflammatory reactions such as redness, swelling and heat pain. PG synthetase inhibitors have good anti-inflammatory effects, and also indicate that PG substances play an important role in inflammation. 5 Tumor metastasis: PGI2 in arterial tissue of patients with malignant tumors is less than that of benign tumor patients, and the half-life is shortened. The production of PGI2 and TXB2 may prevent tumor cells from invading platelets and adhering to the surface of blood vessels. Factors that inhibit platelet TXA2 production and increase vascular endothelial cell PGI2 production are tumor metastasis. Low results may be diseases: diabetes, renal vascular hypertension considerations (1) This method uses a high specific radioactivity [3H]-label, using immuno-binding non-equilibrium method, the sensitivity is high, the minimum detection value is 6.25 ~ 12.5pg / tube. (2) 6-keto-prostaglandin F1α100, 200 pg/ml was added to normal plasma, and the recovery rate was 88.5% to 98.5%, the intra-assay CV was 5.5% to 10.5%, and the inter-assay CV was 10.5% to 15.1%. Inspection process (1) Collecting and extracting test specimens. (2) Take 8mm × 70mm glass test tube, T tube plus PBS 0.4ml, NBS tube plus PBS 0.3ml, S0 plus PBS 0.2ml, S1 ~ 7 plus standard working solution 0.2ml, measuring tube plus sample to be tested 0.2ml. (3) In addition to the T tube and the NSB tube, the other tubes were added with 0.1 ml of antiserum and placed at 4 ° C for 8 to 12 hours. (4) Add 0.1 ml of [3H]-label to all tubes and put it at 4 °C for 8-12 hours. (5) In addition to the T tube, 0.1 ml of each separating agent was added, and the mixture was quickly mixed for 1 min, placed at 4 ° C for 15 min, and centrifuged at 3500 r/min (4 ° C) for 15 min. (6) Pour all the supernatant into the counting bottle with 12ml of scintillation liquid in advance, mix it, put it on the liquid scintillation measuring instrument for 12h, and measure it according to the first programmed procedure, draw the standard curve, relevant parameters and The 6-keto-prostaglandin F1 alpha value of the sample tube. Then convert it to ng/L. Not suitable for the crowd Generally no taboos. Adverse reactions and risks 1. Infection: Pay attention to aseptic operation when collecting blood, pay attention to local cleaning after puncture, prevent water pollution and avoid infection. 2, bleeding: blood collection needle damage to local blood vessels or tissue caused by local bleeding, should try to avoid puncture too deep.